Non-canonical Wnt pathway expression in the developing mouse and human retina.

Autor: Campos RC; The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA; Department of Development, Stem Cells and Regenerative Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA., Matsunaga K; Keck School of Medicine, University of Southern California, Los Angeles, CA, 90033, USA., Reid MW; The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA., Fernandez GE; The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA., Stepanian K; The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA., Bharathan SP; The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA; The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA., Li M; USC Libraries Bioinformatics Services, University of Southern California, Los Angeles, CA, USA., Thornton ME; Maternal-Fetal Medicine Division, Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA., Grubbs BH; Maternal-Fetal Medicine Division, Department of Obstetrics and Gynecology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA., Nagiel A; The Vision Center, Department of Surgery, Children's Hospital Los Angeles, Los Angeles, CA, USA; The Saban Research Institute, Children's Hospital Los Angeles, Los Angeles, CA, USA; Roski Eye Institute, Department of Ophthalmology, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA. Electronic address: anagiel@chla.usc.edu.
Jazyk: angličtina
Zdroj: Experimental eye research [Exp Eye Res] 2024 Jul; Vol. 244, pp. 109947. Date of Electronic Publication: 2024 May 28.
DOI: 10.1016/j.exer.2024.109947
Abstrakt: The non-canonical Wnt pathway is an evolutionarily conserved pathway essential for tissue patterning and development across species and tissues. In mammals, this pathway plays a role in neuronal migration, dendritogenesis, axon growth, and synapse formation. However, its role in development and synaptogenesis of the human retina remains less established. In order to address this knowledge gap, we analyzed publicly available single-cell RNA sequencing (scRNAseq) datasets for mouse retina, human retina, and human retinal organoids over multiple developmental time points during outer retinal maturation. We identified ligands, receptors, and mediator genes with a putative role in retinal development, including those with novel or species-specific expression, and validated this expression using fluorescence in situ hybridization (FISH). By quantifying outer nuclear layer (ONL) versus inner nuclear layer (INL) expression, we provide evidence for the differential expression of certain non-canonical Wnt signaling components in the developing mouse and human retina during outer plexiform layer (OPL) development. Importantly, we identified distinct expression patterns of mouse and human FZD3 and WNT10A, as well as previously undescribed expression, such as for mouse Wnt2b in Chat + starburst amacrine cells. Human retinal organoids largely recapitulated the human non-canonical Wnt pathway expression. Together, this work provides the basis for further study of non-canonical Wnt signaling in mouse and human retinal development and synaptogenesis.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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