Cytotoxicity evaluation of Chlorhexidine and Blue®M applied to a human gingival fibroblast (HGF-1) and keratinocytes (NOK-SI): In vitro study.

Autor: Cunha G; Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery, São Paulo State University (Unesp), School of Dentistry, Araraquara, Brazil; Private Practice. Louveira, Brazil. Electronic address: giovanni.cunha@unesp.br., D'Angieri Saugo G; Private Practice., Gabrielli MAC; Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery, São Paulo State University (Unesp), School of Dentistry, Araraquara, Brazil., Barbeiro CO; Oral Medicine, Department of Diagnosis and Surgery, São Paulo State University (Unesp), School of Dentistry, Araraquara, SP, Brazil., de Almeida LY; Oral Medicine, Department of Diagnosis and Surgery, São Paulo State University (Unesp), School of Dentistry, Araraquara, SP, Brazil., Bufalino A; Department of Diagnosis and Surgery, São Paulo State University (Unesp), School of Dentistry, Araraquara, SP, Brazil., Pereira-Filho VA; Department of Diagnosis and Surgery, Division of Oral and Maxillofacial Surgery, São Paulo State University (Unesp), School of Dentistry, Araraquara, Brazil.
Jazyk: angličtina
Zdroj: Journal of stomatology, oral and maxillofacial surgery [J Stomatol Oral Maxillofac Surg] 2024 Oct; Vol. 125 (5S2), pp. 101923. Date of Electronic Publication: 2024 May 28.
DOI: 10.1016/j.jormas.2024.101923
Abstrakt: Chlorhexidine (CHX) is a prime choice to control the oral microbiota. However, it's a chemical agent leading to side effects such as teeth strains, taste disturbance, and desquamation of oral mucosa. Alternatively, the lactoferrin and oxygen-based Blue®M has been introduced as an alternative to the CHX, not disturbing tissue repair. Therefore, the study aimed to evaluate the effects of Blue®M and CHX on oral human fibroblasts (HGF-1) and keratinocytes (NOK-SI). Cell cultures using HGF-1 and NOK-SI evaluated cell proliferation, cell cycle, apoptosis and necrosis, and migration. In the dose-effect test, Blue®M reduced the HGF-1 sample in a 4-fold concentration than CHX (CHX: 173.07 ±10.27; Blue®M: 43.86 ±3.04). The proliferation test revealed an eightfold reduction of the sample for CHX, while for Blue®M, the proliferation rate was eighteen times lower. The apoptosis and necrosis rates increased by 25% (p<0.0001) for HGF-1 for both substances. In NOK-SI, the apoptosis rates increased by 10% (p=0.02) and 15% (p=0.001) for CHX and Blue®M, respectively. Furthermore, the fibroblast had a lower capacity for wound closure in the Scratch Assay (monolayer cell migration) for Blue®M. Despite the limitations of this in vitro study, the results of the lactoferrin and oxygen-based Blue®M demonstrated cytotoxicity in doses over the Minimum inhibitory concentration and Minimum bactericidal concentration for Oral fibroblasts (HGF- 1) and Keratinocytes (NOK-SI).
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Masson SAS. All rights reserved.)
Databáze: MEDLINE
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