Aptameric photonic structure-based optical biosensor for the detection of microcystin.

Autor: Hussain S; Department of Chemistry, College of Science, Alfaisal University, Al-Maather, 11533, Riyadh, Saudi Arabia., Al-Tabban A; Department of Chemistry, College of Science, Alfaisal University, Al-Maather, 11533, Riyadh, Saudi Arabia., Zourob M; Department of Chemistry, College of Science, Alfaisal University, Al-Maather, 11533, Riyadh, Saudi Arabia. Electronic address: mzourob@alfaisal.edu.
Jazyk: angličtina
Zdroj: Biosensors & bioelectronics [Biosens Bioelectron] 2024 Sep 15; Vol. 260, pp. 116413. Date of Electronic Publication: 2024 May 21.
DOI: 10.1016/j.bios.2024.116413
Abstrakt: An optical photonic biosensor for the detection of microcystin (MC) has been developed using an aptamer-immobilized interpenetrating polymeric network (IPN aptamer ) intertwined with solid-state cholesteric liquid crystals (CLC solid s). The IPN was constructed with a polyacrylic acid hydrogel (PAA). Aptamer immobilization enhances polarity while blocking hydrogen bonding between the carboxylic groups of PAA-IPN hydrogel, thereby increasing the swelling ratio of the PAA-IPN hydrogel. This leads to an expansion in the helical pitch of the corresponding IPN aptamer -CLC solid biosensor chip and results in a red-shift in the reflected color. Upon exposure to an aqueous MC solution, the IPN aptamer -CLC solid biosensor chip exhibits aptamer-mediated engulfment of MC, resulting in reduced polarity of the IPN aptamer complex and a consequential blue-shift in the biosensor chip color occurred. The wavelength shift of the IPN aptamer -CLC solid biosensor chip demonstrates a linear change with an increase in MC concentration from 3.8 to 150 nM, with a limit of detection of 0.88 nM. This novel optical biosensor is characterized by its low cost, simplicity, selectivity, and sensitivity, offering a promising strategy for designing similar toxin biosensors through the modification of biological receptors.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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