Transmission of viable Haemophilus ducreyi by Musca domestica.

Autor: Stabile HD; Department of Biology, Allegheny College, Meadville, Pennsylvania, United States of America.; Lake Erie College of Osteopathic Medicine, Erie, Pennsylvania, United States of America., McCandless K; Department of Biology, Allegheny College, Meadville, Pennsylvania, United States of America.; Krystal Biotech, Inc., Pittsburgh, Pennsylvania, United States of America., Donlan RA; Department of Biology, Allegheny College, Meadville, Pennsylvania, United States of America.; University of Pittsburgh Medical Center, Department of Microbiology and Molecular Genetics, Pittsburgh, Pennsylvania, United States of America., Gaston JR; Department of Biology, Allegheny College, Meadville, Pennsylvania, United States of America.; University of Pittsburgh Medical Center, St. Margaret Hospital, Pittsburgh, Pennsylvania, United States of America., Humphreys TL; Department of Biology, Allegheny College, Meadville, Pennsylvania, United States of America.
Jazyk: angličtina
Zdroj: PLoS neglected tropical diseases [PLoS Negl Trop Dis] 2024 May 30; Vol. 18 (5), pp. e0012194. Date of Electronic Publication: 2024 May 30 (Print Publication: 2024).
DOI: 10.1371/journal.pntd.0012194
Abstrakt: Haemophilus ducreyi was historically known as the causative agent of chancroid, a sexually-transmitted disease causing painful genital ulcers endemic in many low/middle-income nations. In recent years the species has been implicated as the causative agent of nongenital cutaneous ulcers affecting children of the South Pacific Islands and West African countries. Much is still unknown about the mechanism of H. ducreyi transmission in these areas, and recent studies have identified local insect species, namely flies, as potential transmission vectors. H. ducreyi DNA has been detected on the surface and in homogenates of fly species sampled from Lihir Island, Papua New Guinea. The current study develops a model system using Musca domestica, the common house fly, as a model organism to demonstrate proof of concept that flies are a potential vector for the transmission of viable H. ducreyi. Utilizing a green fluorescent protein (GFP)-tagged strain of H. ducreyi and three separate exposure methods, we detected the transmission of viable H. ducreyi by 86.11% ± 22.53% of flies sampled. Additionally, the duration of H. ducreyi viability was found to be directly related to the bacterial concentration, and transmission of H. ducreyi was largely undetectable within one hour of initial exposure. Push testing, Gram staining, and PCR were used to confirm the identity and presence of GFP colonies as H. ducreyi. This study confirms that flies are capable of mechanically transmitting viable H. ducreyi, illuminating the importance of investigating insects as vectors of cutaneous ulcerative diseases.
Competing Interests: The authors have declared that no competing interests exist.
(Copyright: © 2024 Stabile et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
Databáze: MEDLINE
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