Activation of Ventral Tegmental Area Dopaminergic Neurons Projecting to the Parabrachial Nucleus Promotes Emergence from Propofol Anesthesia in Male Rats.

Autor: Jia L; Department of Anesthesiology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China.; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China., Yin J; Department of Anesthesiology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China.; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China., Liu T; Department of Anesthesiology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China.; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China., Qi W; Department of Anesthesiology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China.; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China., Du T; Department of Anesthesiology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China.; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China., Li Q; Department of Anesthesiology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China.; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China., Ma K; Department of Physiology, School of Medicine, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University, Shihezi, China., Si J; Department of Physiology, School of Medicine, Key Laboratory of Xinjiang Endemic and Ethnic Diseases, Shihezi University, Shihezi, China., Yin J; Department of Anesthesiology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China. yiw6654328@163.com.; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China. yiw6654328@163.com., Li Y; Department of Anesthesiology, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China. 1249623003@qq.com.; NHC Key Laboratory of Prevention and Treatment of Central Asia High Incidence Diseases, First Affiliated Hospital, School of Medicine, Shihezi University, Shihezi, China. 1249623003@qq.com.
Jazyk: angličtina
Zdroj: Neurochemical research [Neurochem Res] 2024 Aug; Vol. 49 (8), pp. 2060-2074. Date of Electronic Publication: 2024 May 30.
DOI: 10.1007/s11064-024-04169-x
Abstrakt: Since the clinical introduction of general anesthesia, its underlying mechanisms have not been fully elucidated. The ventral tegmental area (VTA) and parabrachial nucleus (PBN) play pivotal roles in the mechanisms underlying general anesthesia. However, whether dopaminergic (DA) projections from the VTA to the PBN play a role in mediating the effects of general anesthesia is unclear. We microinjected 6-hydroxydopamine into the PBN to damage tyrosine hydroxylase positive (TH+) neurons and found a prolonged recovery time from propofol anesthesia. We used calcium fiber photometry recording to explore the activity of TH + neurons in the PBN. Then, we used chemogenetic and optogenetic approaches either activate the VTA DA -PBN pathway, shortening the propofol anesthesia emergence time, or inhibit this pathway, prolonging the emergence time. These data indicate the crucial involvement of TH + neurons in the PBN in regulating emergence from propofol anesthesia, while the activation of the VTA DA -PBN pathway facilitates the emergence of propofol anesthesia.
(© 2024. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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