Characterisation of common hypothetical surface peptides between protozoan parasites (Perkinsus olseni) originating from different geographical locations.

Autor:	Hanrio E; The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia.; Department of Primary Industries and Regional Development, Government of Western Australia, Perth, WA 6000, Australia., Bogema D; Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Menangle, NSW 2568, Australia., Davern K; Monoclonal Antibody Facility, QEII Medical Centre, 6 Verdun St, Nedlands, WA 6009, Australia., Batley J; The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia., Clarke M; Metabolomics Australia, Centre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, 35 Stirling Hwy, Nedlands WA 6009, Australia., Abudulai L; Metabolomics Australia, Centre for Microscopy, Characterisation and Analysis (CMCA), The University of Western Australia, 35 Stirling Hwy, Nedlands WA 6009, Australia., Severn-Ellis A; Department of Primary Industries and Regional Development, Government of Western Australia, Perth, WA 6000, Australia., Dang C; The University of Western Australia, 35 Stirling Hwy, Crawley, WA 6009, Australia.; Department of Primary Industries and Regional Development, Government of Western Australia, Perth, WA 6000, Australia.
Jazyk:	angličtina
Zdroj:	Diseases of aquatic organisms [Dis Aquat Organ] 2024 May 30; Vol. 158, pp. 143-155. Date of Electronic Publication: 2024 May 30.
DOI:	10.3354/dao03790
Abstrakt:	Perkinsus olseni and P. marinus are classified as notifiable pathogens by the World Organisation for Animal Health and are known to cause perkinsosis in a variety of molluscs globally. Mass mortalities due to these parasites in farms and in the wild have been a recurrent issue. Diagnosis for these protozoans is currently done using Ray's fluid thioglycollate medium method followed by optical microscopy or molecular assays. Both require a high level of skill and are time-consuming. An immunoassay method would make the diagnosis of perkinsosis quicker and cheaper. The present study used mass spectrometry-based proteomics to investigate common hypothetical surface peptides between different geographical isolates of P. olseni, which could be used to develop immunoassays in the future. Two peptides were identified: POLS_08089, which is a 42.7 kDa peptide corresponding to the 60S ribosomal subunit protein L4; and POLS_15916, which is a conserved hypothetical protein of 55.6 kDa. The identification of peptides may allow the development of immunoassays through a more targeted approach.
Databáze:	MEDLINE
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