Cryptosporidium Genomics - Current Understanding, Advances, and Applications.

Autor: Agyabeng-Dadzie F; Department of Genetics, University of Georgia, Athens, GA 30602 USA., Xiao R; Institute of Bioinformatics, University of Georgia, Athens, GA 30602 USA., Kissinger JC; Department of Genetics, University of Georgia, Athens, GA 30602 USA.; Institute of Bioinformatics, University of Georgia, Athens, GA 30602 USA.; Center for Tropical and Emerging Global Diseases, University of Georgia, Coverdell Center, 107, 500 D.W. Brooks Drive, Athens, GA 30602 USA.
Jazyk: angličtina
Zdroj: Current tropical medicine reports [Curr Trop Med Rep] 2024; Vol. 11 (2), pp. 92-103. Date of Electronic Publication: 2024 Mar 23.
DOI: 10.1007/s40475-024-00318-y
Abstrakt: Purpose of Review: Here we highlight the significant contribution that genomics-based approaches have had on the field of Cryptosp oridium research and the insights these approaches have generated into Cryptosporidium biology and transmission.
Recent Findings: There are advances in genomics, genetic manipulation, gene expression, and single-cell technologies. New and better genome sequences have revealed variable sub-telomeric gene families and genes under selection. RNA expression data now include single-cell and post-infection time points. These data have provided insights into the Cryptosporidium life cycle and host-pathogen interactions. Antisense and ncRNA transcripts are abundant. The critical role of the dsRNA virus is becoming apparent.
Summary: The community's ability to identify genomic targets in the abundant, yet still lacking, collection of genomic data, combined with their increased ability to assess function via gene knock-out, is revolutionizing the field. Advances in the detection of virulence genes, surveillance, population genomics, recombination studies, and epigenetics are upon us.
Competing Interests: Competing interestsJCK owns PacBio stock.
(© The Author(s) 2024.)
Databáze: MEDLINE
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