Outcome of stress on G protein-coupled receptors and hypoxia inducible factor-1α.

Autor: Reséndiz-Albor AA; Laboratorio de Inmunidad de Mucosas, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Ciudad de México, México., Arciniega-Martínez IM; Laboratorio de Inmunidad de Mucosas, Escuela Superior de Medicina, Instituto Politécnico Nacional, Plan de San Luis y Díaz Mirón, Ciudad de México, México., Montes de Oca AC; Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina Instituto Politecnico Nacional Plan de San Luis y Salvador Diaz Miron, Ciudad de México, México., Guzmán-Mejía F; Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana, Unidad Xochimilco, Ciudad de México, México., Drago-Serrano ME; Departamento de Sistemas Biológicos, Universidad Autónoma Metropolitana, Unidad Xochimilco, Ciudad de México, México., Estrada-Jiménez T; Facultad de Medicina, Decanato de Ciencias Médicas, Universidad Popular Autónoma de Estado de Puebla, Ciudad de México, México., Abarca-Rojano E; Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina Instituto Politecnico Nacional Plan de San Luis y Salvador Diaz Miron, Ciudad de México, México.
Jazyk: angličtina
Zdroj: Journal of medicine and life [J Med Life] 2024 Feb; Vol. 17 (2), pp. 201-204.
DOI: 10.25122/jml-2023-0363
Abstrakt: Stress drives neuroendocrine signals with detrimental effects to the intestinal homeostasis. The aim of this study was to evaluate the effect of stress on intestinal hypoxia response elements, including G protein-coupled receptor 41 (GPR41), GPR43, and hypoxia inducible factor (HIF)-1α. Groups of five BALB/c mice were subjected to acute (2 h per day) and chronic (2 h per day for 4 days) stress induced by restraint, and the results were compared to those of an unstressed control group. Whole mucosal samples from the colon were collected to evaluate the expression of GPR41, GPR43 and HIF-1α using Western blot chemiluminescent analysis. Compared to the control group, in the chronic stress group the expression of GPR43 ( P = 0.0092) and HIF-1α ( P < 0.0001) were significantly lower and the expression of GPR41 was similar ( P = 0.9184); acute stress significantly increased HIF-1α expression ( P = 0.0030) and increased GPR41 expression ( P = 0.0937), without affecting GPR43 ( P = 0.9184). These findings offer insights into the modulation of hypoxia response elements under stress conditions and their pharmacological implications for developing drugs that mitigate the effects of stress on intestinal homeostasis.
Competing Interests: The authors declare no conflict of interest.
(© 2024 by the authors.)
Databáze: MEDLINE
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