Investigation of the vaccine potential of an in silico designed FepA peptide vaccine against Shigella flexneri in mice model.

Autor:	Ali MR; Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh., Mahmud S; Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh., Faruque MO; Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh., Hossain MI; Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh., Hossain MA; Florey Institute of Neuroscience and Mental Health, University of Melbourne, Victoria 3052, Australia., Kibria KMK; Department of Biotechnology and Genetic Engineering, Mawlana Bhashani Science and Technology University, Tangail-1902, Bangladesh.
Jazyk:	angličtina
Zdroj:	Vaccine: X [Vaccine X] 2024 May 08; Vol. 18, pp. 100493. Date of Electronic Publication: 2024 May 08 (Print Publication: 2024).
DOI:	10.1016/j.jvacx.2024.100493
Abstrakt:	Background: Shigellosis is one of the significant causes of diarrhea in Bangladesh. It is a global health problem; approximately 1.3 million people die yearly from Shigellosis. The current treatment method, using different antibiotics against Shigellosis is ineffective. Moreover, it becomes a worrying situation due to the emergence of antibiotic-resistant pathogenic microbes responsible for these diarrheal diseases. Methodology: Previous immunoinformatics study predicted a potential peptide from the Ferric enterobactin protein (FepA) of Shigella spp. In this study, we have chemically synthesized the FepA peptide. As a highly immunogenic, FepA peptide conjugated with KLH has been tested in mice model with complete and incomplete adjuvants as a vaccine candidate. Results: Immunological analysis showed that all vaccinated mice were immunologically boosted, which was statistically significant ( P- value 0.0325) compared to control mice. Immunological analysis for bacterial neutralization test result was also statistically significant ( P -value 0.0468), where each ELISA plate was coated with 1 × 10 7 S. flexneri cells. The Challenge test with 1 × 10 12 S. flexneri cells to each vaccinated and controlled mice showed that 37.5 % of control (non-vaccinated) mice died within seven days after the challenge was given while 100 % of vaccinated mice remained strong and stout. The analyses of the post-challenge weight loss of the mice were also significant ( P -value 0.0367) as the weight loss percentage in control mice was much higher than in the vaccinated mice. The pathological and phenotypic appearances of vaccinated mice were also clearly differentiable compared with control mice. Thus all these immunological analysis and pathological appearances directly supported our FepA peptide as a potential immune booster. Conclusion: This study provides evidence that the FepA peptide is a highly immunogenic vaccine candidate against S. flexneri . Therefore, these findings inspire future trials for the evaluation of the suitability of this vaccine candidate against Shigellosis. Competing Interests: The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: [K. M. Kaderi Kibria reports financial support was provided by Government of the People’s Republic of Bangladesh Ministry of Education. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.]. (© 2024 The Author(s).)
Databáze:	MEDLINE
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