Improvements of weaned pigs barn hygiene to reduce the spread of antimicrobial resistance.
| Autor: | Jaleta M; Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Potsdam, Germany.; Dahlem Research School, Freie Universität Berlin, Berlin, Germany., Junker V; Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany., Kolte B; Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.; Technical University Braunschweig, Institute of Microbiology, Braunschweig, Germany., Börger M; Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg, Germany., Werner D; Leibniz Centre for Agricultural Landscape Research (ZALF), Müncheberg, Germany., Dolsdorf C; Teaching and Research Station for Animal Breeding and Husbandry (LVAT), Ruhlsdorf, Germany., Schwenker J; Faculty of Agricultural and Nutritional Sciences Christian-Albrechts-University of Kiel, Kiel, Germany., Hölzel C; Faculty of Agricultural and Nutritional Sciences Christian-Albrechts-University of Kiel, Kiel, Germany., Zentek J; Institute of Animal Nutrition, Free University Berlin, Berlin, Germany., Amon T; Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Potsdam, Germany.; Institute for Animal Hygiene and Environmental Health (ITU), Free University Berlin, Berlin, Germany., Nübel U; Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Braunschweig, Germany.; Technical University Braunschweig, Institute of Microbiology, Braunschweig, Germany.; German Center for Infection Research (DZIF), Partner Site Braunschweig-Hannover, Braunschweig, Germany., Kabelitz T; Leibniz Institute for Agricultural Engineering and Bioeconomy (ATB), Potsdam, Germany. |
|---|---|
| Jazyk: | angličtina |
| Zdroj: | Frontiers in microbiology [Front Microbiol] 2024 May 14; Vol. 15, pp. 1393923. Date of Electronic Publication: 2024 May 14 (Print Publication: 2024). |
| DOI: | 10.3389/fmicb.2024.1393923 |
| Abstrakt: | The spread of antimicrobial resistance (AMR) in animal husbandry is usually attributed to the use of antibiotics and poor hygiene and biosecurity. We therefore conducted experimental trials to improve hygiene management in weaned pig houses and assessed the impact on the spread. For each of the two groups examined, the experimental group (EG) and the control group (CG), three replicate batches of piglets from the same pig breeder, kept in pre-cleaned flat decks, were analyzed. In the flat decks of the experimental groups, the hygiene conditions (cleaning, disinfection, dust removal and fly control) were improved, while regular hygiene measures were carried out in the control groups. The occurrence and spread of AMR were determined in Escherichia coli ( E. coli ; resistance indicator) using cultivation-dependent (CFU) and -independent (qPCR) methods as well as whole genome sequencing of isolates in samples of various origins, including feces, flies, feed, dust and swabs. Surprisingly, there were no significant differences ( p > 0.05) in the prevalence of resistant E. coli between the flat decks managed with conventional techniques and those managed with improved techniques. Selective cultivation delivered ampicillin- and sulfonamide-resistant E. coli proportions of up to 100% and 1.2%, respectively. While 0.5% E. coli resistant to cefotaxime and no ciprofloxacin resistance were detected. There was a significant difference ( p < 0.01) in the abundance of the bla Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Jaleta, Junker, Kolte, Börger, Werner, Dolsdorf, Schwenker, Hölzel, Zentek, Amon, Nübel and Kabelitz.) |
| Databáze: | MEDLINE |
| Externí odkaz: |
|