Two lysin motif extracellular (LysMe) proteins are deployed in rice to facilitate arbuscular mycorrhizal symbiosis.

Autor: Tian L; Department of Biology, School of Life Sciences, Nanjing University, Nanjing, 210023, China., Hao YM; Department of Biology, School of Life Sciences, Nanjing University, Nanjing, 210023, China., Guo R; Department of Biology, School of Life Sciences, Nanjing University, Nanjing, 210023, China., Guo HR; National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai, 200032, China., Cheng JF; Department of Biology, School of Life Sciences, Nanjing University, Nanjing, 210023, China., Liu TR; Department of Biology, School of Life Sciences, Nanjing University, Nanjing, 210023, China., Liu H; Department of Biology, School of Life Sciences, Nanjing University, Nanjing, 210023, China., Lu G; Department of Biology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.; School of Life Sciences, Huaiyin Normal University, Huaian, 223300, China., Wang B; Department of Biology, School of Life Sciences, Nanjing University, Nanjing, 210023, China.
Jazyk: angličtina
Zdroj: The New phytologist [New Phytol] 2024 Jul; Vol. 243 (2), pp. 720-737. Date of Electronic Publication: 2024 May 29.
DOI: 10.1111/nph.19873
Abstrakt: During arbuscular mycorrhizal (AM) symbiosis, plant innate immunity is modulated to a prime state to allow for fungal colonization. The underlying mechanisms remain to be further explored. In this study, two rice genes encoding LysM extracellular (LysMe) proteins were investigated. By obtaining OsLysMepro:GUS transgenic plants and generating oslysme1, oslysme2 and oslysme1oslysme2 mutants via CRISPR/Cas9 technique, OsLysMe genes were revealed to be specifically induced in the arbusculated cells and mutations in either gene caused significantly reduced root colonization rate by AM fungus Rhizophagus irregularis. Overexpression of OsLysMe1 or OsLysMe2 dramatically increased the colonization rates in rice and Medicago truncatula. The electrophoretic mobility shift assay and dual-luciferase reporter assay supported that OsLysMe genes are regulated by OsWRI5a. Either OsLysMe1 or OsLysMe2 can efficiently rescue the impaired AM phenotype of the mtlysme2 mutant, supporting a conserved function of LysMe across monocotyledonous and dicotyledonous plants. The co-localization of OsLysMe proteins with the apoplast marker SP-OsRAmy3A implies their probable localization to the periarbuscular space (PAS) during symbiosis. Relative to the fungal biomass marker RiTEF, some defense-related genes showed disproportionately high expression levels in the oslysme mutants. These data support that rice plants deploy two OsLysMe proteins to facilitate AM symbiosis, likely by diminishing plant defense responses.
(© 2024 The Authors. New Phytologist © 2024 New Phytologist Foundation.)
Databáze: MEDLINE
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