Injectable PEG Hydrogels with Tissue-Like Viscoelasticity Formed through Reversible Alendronate-Calcium Phosphate Crosslinking for Cell-Material Interactions.

Autor: Yu H; Centre for Craniofacial and Regenerative Biology, King's College London, London, UK., Yan Z; Centre for Craniofacial and Regenerative Biology, King's College London, London, UK., Dreiss CA; Institute of Pharmaceutical Science, King's College London, London, SE1 9NH, UK., Gaitano GG; Department of Chemistry, University of Navarra, Pamplona, 31080, Spain., Jarvis JA; Randall Division of Cell and Molecular Biophysics and NMR Facility, Centre for Biomolecular Spectroscopy, King's College London, London, SE1 1UL, UK., Gentleman E; Centre for Craniofacial and Regenerative Biology, King's College London, London, UK., da Silva RMP; Centre for Craniofacial and Regenerative Biology, King's College London, London, UK., Grigoriadis AE; Centre for Craniofacial and Regenerative Biology, King's College London, London, UK.
Jazyk: angličtina
Zdroj: Advanced healthcare materials [Adv Healthc Mater] 2024 Sep; Vol. 13 (22), pp. e2400472. Date of Electronic Publication: 2024 Jun 14.
DOI: 10.1002/adhm.202400472
Abstrakt: Synthetic hydrogels provide controllable 3D environments, which can be used to study fundamental biological phenomena. The growing body of evidence that cell behavior depends upon hydrogel stress relaxation creates a high demand for hydrogels with tissue-like viscoelastic properties. Here, a unique platform of synthetic polyethylene glycol (PEG) hydrogels in which star-shaped PEG molecules are conjugated with alendronate and/or RGD peptides, attaining modifiable degradability as well as flexible cell adhesion, is created. Novel reversible ionic interactions between alendronate and calcium phosphate nanoparticles, leading to versatile viscoelastic properties with varying initial elastic modulus and stress relaxation time, are identified. This new crosslinking mechanism provides shear-thinning properties resulting in differential cellular responses between cancer cells and stem cells. The novel hydrogel system is an improved design to the other ionic crosslink platforms and opens new avenues for the development of pathologically relevant cancer models, as well as minimally invasive approaches for cell delivery for potential regenerative therapies.
(© 2024 The Author(s). Advanced Healthcare Materials published by Wiley‐VCH GmbH.)
Databáze: MEDLINE
Externí odkaz: