Evaluation of commercial immunodiffusion reagents for detecting serum anti-Paracoccidioides antibodies.
| Autor: | Cognialli RCR; Universidade Federal do Paraná, Hospital de Clínicas, Curitiba, PR, Brasil., Caceres DH; Center of Expertise in Mycology Radboudumc/CWZ. Nijmegen, the Netherlands.; Universidad del Rosario, Studies in Translational Microbiology and Faculty of Medical Sciences, Emerging Diseases Research Group. Bogota, Colombia.; IMMY. Norma, OK, United States of America., Telles FQ; Universidade Federal do Paraná, Hospital de Clínicas, Departamento de Saúde Coletiva, Curitiba, PR, Brasil. |
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| Jazyk: | angličtina |
| Zdroj: | Revista da Sociedade Brasileira de Medicina Tropical [Rev Soc Bras Med Trop] 2024 May 27; Vol. 57, pp. e007052024. Date of Electronic Publication: 2024 May 27 (Print Publication: 2024). |
| DOI: | 10.1590/0037-8682-0094-2024 |
| Abstrakt: | Background: Accurate diagnosis of paracoccidioidomycosis is crucial for improving patient outcomes. Paracoccidioides antibody detection by double immunodiffusion (DID) is a convenient diagnostic tool, but testing performance can vary based on certain factors. Methods: We assessed DID performance using a commercially prepared Paracoccidioides reagents (IMMY, USA), involving 40 serum specimens, including 20 from patients with proven paracoccidioidomycosis and 20 from patients without the disease. The DID test demonstrated a sensitivity of 90% (95% CI=68%-99%) and a specificity of 100% (95% CI=83%-100%). Conclusions: Our findings suggest that DID using commercial reagents may provide a feasible tool with satisfactory testing performance for anti-Paracoccidioides antibody detection. |
| Databáze: | MEDLINE |
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