Pan-cortical 2-photon mesoscopic imaging and neurobehavioral alignment in awake, behaving mice.
| Autor: | Vickers ED; Institute of Neuroscience, University of Oregon, Eugene, United States., McCormick DA; Institute of Neuroscience, University of Oregon, Eugene, United States.; Department of Biology, University of Oregon, Eugene, United States. |
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| Jazyk: | angličtina |
| Zdroj: | ELife [Elife] 2024 May 29; Vol. 13. Date of Electronic Publication: 2024 May 29. |
| DOI: | 10.7554/eLife.94167 |
| Abstrakt: | The flow of neural activity across the neocortex during active sensory discrimination is constrained by task-specific cognitive demands, movements, and internal states. During behavior, the brain appears to sample from a broad repertoire of activation motifs. Understanding how these patterns of local and global activity are selected in relation to both spontaneous and task-dependent behavior requires in-depth study of densely sampled activity at single neuron resolution across large regions of cortex. In a significant advance toward this goal, we developed procedures to record mesoscale 2-photon Ca 2+ imaging data from two novel in vivo preparations that, between them, allow for simultaneous access to nearly all 0f the mouse dorsal and lateral neocortex. As a proof of principle, we aligned neural activity with both behavioral primitives and high-level motifs to reveal the existence of large populations of neurons that coordinated their activity across cortical areas with spontaneous changes in movement and/or arousal. The methods we detail here facilitate the identification and exploration of widespread, spatially heterogeneous neural ensembles whose activity is related to diverse aspects of behavior. Competing Interests: EV, DM No competing interests declared (© 2024, Vickers and McCormick.) |
| Databáze: | MEDLINE |
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