| Autor: |
Shin MR; Department of Herbology, College of Korean Medicine, Daegu Haany University, Daegu, Republic of Korea., Kim MJ; Department of Herbology, College of Korean Medicine, Daegu Haany University, Daegu, Republic of Korea.; Research Center for Herbal Convergence on Liver Disease, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, Republic of Korea., Lee JA; Department of Herbology, College of Korean Medicine, Daegu Haany University, Daegu, Republic of Korea., Lee ES; Department of Herbal Crop Research, National Institute of Horticultural & Herbal Science, Eumseong, Republic of Korea., Park HJ; DHU Bio Convergence Testing Center, Daegu Haany University, Gyeongsan-si, Gyeongsangbuk-do, Republic of Korea., Roh SS; Department of Herbology, College of Korean Medicine, Daegu Haany University, Daegu, Republic of Korea. |
| Abstrakt: |
Lipolysis is the hydrolysis of triglycerides (TGs), commonly known as fats. Intracellular lipolysis of TG is associated with adipose triglyceride lipase (ATGL), which provides fatty acids during times of metabolic need. The aim of this study was to determine whether Coix lacryma-jobi L. var. ma-yuen Stapf ( Coix ) sprouts (CS) can alleviate obesity through lipolysis. Overall, we investigated the potential of CS under in vitro and in vivo conditions and confirmed the underlying mechanisms. Huh7 cells were exposed to free fatty acids (FFAs), and C57BL/6J mice were fed a 60% high-fat diet. When FFA were introduced into Huh7 cells, the intracellular TG levels increased within the Huh7 cells. However, CS treatment significantly reduced intracellular TG levels. Furthermore, CS decreased the expression of Pparγ and Srebp1c mRNA and downregulated the mutant Pnpla3 (I148M) mRNA. Notably, CS significantly upregulated ATGL expression. CS treatment at a dose of 200 mg/kg/day resulted in a significant and dose-dependent decrease in body weight gain and epididymal adipose tissue weight. Specifically, the group treated with CS (200 mg/kg/day) exhibited a significant modulation of serum lipid biomarkers. In addition, CS ameliorated histological alterations in both the liver and adipose tissues. In summary, CS efficiently inhibited lipid accumulation through the activation of the lipolytic enzyme ATGL coupled with the suppression of enzymes involved in TG synthesis. Consequently, CS show promise as a potential anti-obesity agent. |
