Anti-inflammatory effect of Faloak ( Sterculia quadrifida R. Br) stem bark on TNF-α, IL-1β, and IL-6 in DENV-3-infected Wistar rats.

Autor: Riwu AG; Doctoral Program of Medical Science, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia., Nugraha J; Department of Clinical Pathology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia., Triyono EA; Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia., Purwanto DA; Department of Pharmaceutical Sciences, Faculty of Pharmacy, Universitas Airlangga, Surabaya, Indonesia.
Jazyk: angličtina
Zdroj: Open veterinary journal [Open Vet J] 2024 Apr; Vol. 14 (4), pp. 1043-1050. Date of Electronic Publication: 2024 Apr 30.
DOI: 10.5455/OVJ.2024.v14.i4.11
Abstrakt: Background: Dengue infection can trigger an immunological response that results in an inflammatory reaction, which acts as a defensive mechanism to protect the host. Dengue infection leads to an elevation in the release of pro-inflammatory cytokines, including tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), and interleukin-6 (IL-6). These three cytokines have been shown to correlate with the development of thrombocytopenia and plasma leakage, which is related to the severity of the disease.
Aim: This study aims to investigate the effect of faloak ( Sterculia quadrifida R. Br) stem bark on TNF-α, IL-1β, and IL-6 levels in Wistar rats infected with dengue, specifically DENV-3.
Methods: A group of 27 male Wistar rats ( Rattus norvegicus ) aged 2-3 months and weighting 200-300 g were divided into three distinct groups: healthy, dengue, and treatment (dengue infection and extract) groups. The rats in both the dengue and treatment groups were administered an injection of DENV-3 with a titer of 105 pfu at a dosage of 0.8 cc via the intraperitoneal route. The propagation of DENV-3 was initiated using C6/36 cells, and it underwent four passages. The extract was administered orally via a nasogastric tube at a dosage of 1,500 mg/kg body weight once daily for 7 days. The healthy group underwent blood sampling on the first day, whereas the dengue and therapy groups underwent blood sampling on the fifth and eighth, respectively.
Results: Compared with the healthy group, TNF-α levels in the dengue and treatment groups showed significant differences on day 5 post-infection. The post hoc analysis revealed a statistically significant difference between the dengue-treatment and dengue-healthy groups. The IL-1β levels in the dengue and healthy groups significantly differed on days 5 and 8 post-infection compared to the healthy group. The treatment group had less of a decrease in IL-6 levels on days 5 and 8 than the dengue group. However, no statistically significant differences were observed.
Conclusion: The stem bark of S. quadrifida shows potential as an anti-inflammatory agent in dengue infections, particularly in its ability to decrease levels of TNF-α and IL-1β.
Competing Interests: There is no conflict of interest in this study.
Databáze: MEDLINE