Delayed repair of the facial nerve and its negative impacts on nerve and muscle regeneration.

Autor:	Bueno CRS; Department of Biological Sciences, Bauru School of Dentistry (FOB), University of São Paulo (USP), Bauru, SP, Brazil.; Dentistry School, University Center of Adamantina (UNIFAI), Adamantina, SP, Brazil.; Medical School, University Center of Adamantina (UNIFAI), Adamantina, SP, Brazil., Buchaim DV; Medical School, University Center of Adamantina (UNIFAI), Adamantina, SP, Brazil.; Graduate Program in Structural and Functional Interactions in Rehabilitation, University of Marilia (UNIMAR), Marília, SP, Brazil.; Graduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science (FMVZ), University of São Paulo (USP), São Paulo, SP, Brazil., Barraviera B; Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu, SP, Brazil.; Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, Brazil., Ferreira RS Jr; Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu, SP, Brazil.; Graduate Program in Tropical Diseases, Botucatu Medical School (FMB), São Paulo State University (UNESP), Botucatu, SP, Brazil., Santos PSDS; Department of Surgery, Stomatology, Pathology and Radiology, Bauru School of Dentistry (FOB), University of São Paulo (USP), Bauru, SP, Brazil., Reis CHB; Department of Biological Sciences, Bauru School of Dentistry (FOB), University of São Paulo (USP), Bauru, SP, Brazil.; Graduate Program in Structural and Functional Interactions in Rehabilitation, University of Marilia (UNIMAR), Marília, SP, Brazil.; UNIMAR Beneficent Hospital (HBU), University of Marilia (UNIMAR), Marília, SP, Brazil., Cini MA; Medical School, University of West Paulista (UNOESTE), Guarujá, SP, Brazil., Kuga MC; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, SP, Brazil., Rosa GM; Dentistry School, Faculty of the Midwest Paulista (FACOP), Piratininga, SP, Brazil., Buchaim RL; Department of Biological Sciences, Bauru School of Dentistry (FOB), University of São Paulo (USP), Bauru, SP, Brazil.; Graduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science (FMVZ), University of São Paulo (USP), São Paulo, SP, Brazil.
Jazyk:	angličtina
Zdroj:	The journal of venomous animals and toxins including tropical diseases [J Venom Anim Toxins Incl Trop Dis] 2024 May 27; Vol. 30, pp. e20230093. Date of Electronic Publication: 2024 May 27 (Print Publication: 2024).
DOI:	10.1590/1678-9199-JVATITD-2023-0093
Abstrakt:	Background: In this experimental protocol, we evaluated the immediate and delayed repair of the buccal branch of the facial nerve (BBFN) with heterologous fibrin biopolymer (HFB) as a coaptation medium and the use of photobiomodulation (PBM), performing functional and histomorphometric analysis of the BBFN and perioral muscles. Methods: Twenty-eight rats were divided into eight groups using the BBFN bilaterally (the left nerve was used for PBM), namely: G1 - control group, right BBFN (without injury); G2 - control group, left BBFN (without injury + PBM); G3 - Denervated right BBFN (neurotmesis); G4 - Denervated left BBFN (neurotmesis + PBM); G5 - Immediate repair of right BBFN (neurotmesis + HFB); G6 - Immediate repair of left BBFN (neurotmesis + HFB + PBM); G7 - Delayed repair of right BBFN (neurotmesis + HFB); G8 - Delayed repair of left BBFN (neurotmesis + HFB + PBM). Delayed repair occurred after two weeks of denervation. All animals were sacrificed after six weeks postoperatively. Results: In the parameters of the BBFN, we observed inferior results in the groups with delayed repair, in relation to the groups with immediate repair, with a significant difference ( p < 0.05) in the diameter of the nerve fiber, the axon, and the thickness of the myelin sheath of the group with immediate repair with PBM compared to the other experimental groups. In measuring the muscle fiber area, groups G7 (826.4 ± 69.90) and G8 (836.7 ± 96.44) were similar to G5 (882.8 ± 70.51). In the functional analysis, the G7 (4.10 ± 0.07) and G8 (4.12 ± 0.08) groups presented normal parameters. Conclusion: We demonstrated that delayed repair of BBFN is possible with HFB, but with worse results compared to immediate repair, and that PBM has a positive influence on nerve regeneration results in immediate repair. Competing Interests: Competing interests: The heterologous fibrin biopolymer (HFB) was provided by the Center for the Study of Venoms and Venomous Animals (CEVAP), São Paulo State University (UNESP), Botucatu, São Paulo, Brazil.
Databáze:	MEDLINE
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