Characterization of Neisseria gonorrhoeae colonization of macrophages under distinct polarization states and nutrients environment.

Autor:	Juárez Rodríguez MD; Department of Molecular and Cellular Physiology, LSUHSC-Shreveport, Louisiana, LA, United States., Marquette M; LSU Health Shreveport, School of Medicine, Louisiana, LA, United States., Youngblood R; Department of Molecular and Cellular Physiology, LSUHSC-Shreveport, Louisiana, LA, United States., Dhungel N; Department of Molecular and Cellular Physiology, LSUHSC-Shreveport, Louisiana, LA, United States., Torres Escobar A; Department of Microbiology and Immunology, LSUHSC-Shreveport, Louisiana, LA, United States., Ivanov SS; Department of Microbiology and Immunology, LSUHSC-Shreveport, Louisiana, LA, United States., Dragoi AM; Department of Molecular and Cellular Physiology, LSUHSC-Shreveport, Louisiana, LA, United States.; Feist-Weiller Cancer Center, LSUHSC-Shreveport, Louisiana, LA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in cellular and infection microbiology [Front Cell Infect Microbiol] 2024 May 14; Vol. 14, pp. 1384611. Date of Electronic Publication: 2024 May 14 (Print Publication: 2024).
DOI:	10.3389/fcimb.2024.1384611
Abstrakt:	Neisseria gonorrhoeae (Ng) is a uniquely adapted human pathogen and the etiological agent of gonorrhea, a sexually transmitted disease. Ng has developed numerous mechanisms to avoid and actively suppress innate and adaptive immune responses. Ng successfully colonizes and establishes topologically distinct colonies in human macrophages and avoids phagocytic killing. During colonization, Ng manipulates the actin cytoskeleton to invade and create an intracellular niche supportive of bacterial replication. The cellular reservoir(s) supporting bacterial replication and persistence in gonorrhea infections are poorly defined. The manner in which gonococci colonize macrophages points to this innate immune phagocyte as a strong candidate for a cellular niche during natural infection. Here we investigate whether nutrients availability and immunological polarization alter macrophage colonization by Ng . Differentiation of macrophages in pro-inflammatory (M1-like) and tolerogenic (M2-like) phenotypes prior to infection reveals that Ng can invade macrophages in all activation states, albeit with lower efficiency in M1-like macrophages. These results suggest that during natural infection, bacteria could invade and grow within macrophages regardless of the nutrients availability and the macrophage immune activation status. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Juárez Rodríguez, Marquette, Youngblood, Dhungel, Torres Escobar, Ivanov and Dragoi.)
Databáze:	MEDLINE
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