Investigation of caliciviruses and astroviruses in Gabonese rodents: A possible influence of national and international trade on the spread of enteric viruses.

Autor: Mbou-Boutambe C; Unité de Recherche en Écologie de la Santé (URES), Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF), Franceville, BP 769, Gabon; Ecole Doctorale Régionale d'Afrique Centrale en Infectiologie Tropicale (EDR), Franceville, BP 876, Gabon. Electronic address: clarkmbou@gmail.com., Mombo IM; Unité Émergence des Maladies Virales, Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF), Franceville, Gabon; Institut de Recherche pour le Développement (IRD), Maladies Infectieuses et Vecteurs, Écologie, Génétique, Évolution et Contrôle (MIVEGEC) (Université de Montpellier-IRD 224-CNRS 5290), Montpellier 34394, France., Rougeron V; International Research Laboratory-REHABS, CNRS-Université Lyon 1-Nelson Mandela University, Nelson Mandela University George Campus, George 6531, South Africa., Degrugillier F; Institut de Recherche pour le Développement (IRD), Maladies Infectieuses et Vecteurs, Écologie, Génétique, Évolution et Contrôle (MIVEGEC) (Université de Montpellier-IRD 224-CNRS 5290), Montpellier 34394, France., Gauthier P; CBGP, IRD, CIRAD, INRA, Montpellier SupAgro, Université de Montpellier, Montpellier, France., Makanga B; Institut de Recherche en Écologie Tropicale (IRET/CENAREST), Libreville, BP 13354, Gabon., Ngoubangoye B; Centre de Primatologie, Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF), Franceville, BP 769, Gabon., Leroy EM; Institut de Recherche pour le Développement (IRD), Maladies Infectieuses et Vecteurs, Écologie, Génétique, Évolution et Contrôle (MIVEGEC) (Université de Montpellier-IRD 224-CNRS 5290), Montpellier 34394, France., Prugnolle F; International Research Laboratory-REHABS, CNRS-Université Lyon 1-Nelson Mandela University, Nelson Mandela University George Campus, George 6531, South Africa., Boundenga L; Unité de Recherche en Écologie de la Santé (URES), Centre Interdisciplinaire de Recherches Médicales de Franceville (CIRMF), Franceville, BP 769, Gabon; Département d'Anthropologie, Université de Durham, South Road, Durham DH1 3LE, UK.
Jazyk: angličtina
Zdroj: Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases [Infect Genet Evol] 2024 Aug; Vol. 122, pp. 105607. Date of Electronic Publication: 2024 May 26.
DOI: 10.1016/j.meegid.2024.105607
Abstrakt: Caliciviruses (Caliciviridae) and astroviruses (Astroviridae) are among the leading cause of non-bacterial foodborne disease and gastroenteritis in human. These non-enveloped RNA viruses infect a wide range of vertebrate species including rodents. Rodents are among the most important hosts of infectious diseases globally and are responsible for over 80 zoonotic pathogens that affect humans. Therefore, screening pathogens in rodents will be is necessary to prevent cross-species transmission to prevent zoonotic outbreaks. In the present study, we screened caliciviruses and astroviruses in order to describe their diversity and whether they harbor strains that can infect humans. RNA was then extracted from intestine samples of 245 rodents and retrotranscribed in cDNA to screen caliciviruses and astroviruses by PCRs. All the samples tested negative for caliciviruses and while astroviruses were detected in 18 (7.3%) samples of Rattus rattus species. Phylogenetic analyses based on the RdRp gene showed that all the sequences belonged to Mamastrovirus genus in which they were genetically related to R. rattus related AstVs previously detected in Gabon or in Rattus spp. AstV from Kenya and Asia. These findings suggested that transportation such as land and railway, as well national and international trade, are likely to facilitate spread of AstVs by the dissemination of rodents.
Competing Interests: Declaration of competing interest The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: