Follicular fluid exosome-derived miR-339-5p enhances in vitro maturation of porcine oocytes via targeting SFPQ, a regulator of the ERK1/2 pathway.

Autor: Han Y; Yanbian University, Jilin, Yanji, 133000, China., Zhang J; Yanbian University, Jilin, Yanji, 133000, China., Liang W; Yanbian University, Jilin, Yanji, 133000, China., Lv Y; Yanbian University, Jilin, Yanji, 133000, China., Luo X; Institute of Animal Biotechnology, Jilin Academy of Agricultural Sciences, Jilin, Gongzhuling, 136100, China., Li C; Yanbian University, Jilin, Yanji, 133000, China., Qu X; Yanbian University, Jilin, Yanji, 133000, China., Zhang Y; Yanbian University, Jilin, Yanji, 133000, China., Gu W; Yanbian University, Jilin, Yanji, 133000, China., Chen X; Yanbian University, Jilin, Yanji, 133000, China. Electronic address: 0000008862@ybu.edu.cn., Jin Y; Yanbian University, Jilin, Yanji, 133000, China. Electronic address: yijin@ybu.edu.cn.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2024 Sep 01; Vol. 225, pp. 107-118. Date of Electronic Publication: 2024 May 15.
DOI: 10.1016/j.theriogenology.2024.04.022
Abstrakt: In this study, we aimed to investigate cytoplasmic maturation and miRNA expression of mature oocytes cultured in porcine follicular fluid exosomes. We also examined the effect of miR-339-5p on oocyte maturation. Twenty eight differentially expressed miRNAs were detected using miRNA-seq. We then transfected cumulus oocyte complexes with miR-339-5p mimics and inhibitor during culture. The results showed that exosomes increased endoplasmic reticulum levels and the amount of lipid droplets, and decreased ROS levels, lipid droplet size, and percentage of oocytes with abnormal cortical granule distribution. Overexpressing miR-339-5p significantly decreased cumulus expansion genes, oocyte maturation-related genes, target gene proline/glutamine-rich splicing factor (SFPQ), ERK1/2 phosphorylation levels, oocyte maturation rate, blastocyst rate, and lipid droplet number, but increased lipid droplet size and the ratio of oocytes with abnormal cortical granule distribution. Inhibiting miR-339-5p reversed the decrease observed during overexpression. Mitochondrial membrane potential and ROS levels did not differ significantly between groups. In summary, exosomes promote oocyte cytoplasmic maturation and miR-339-5p regulating ERK1/2 activity through SFPQ expression, thereby elevating oocyte maturation and blastocyst formation rate in vitro.
Competing Interests: Declaration of competing interest The authors declare that have no competing interests.
(Copyright © 2024. Published by Elsevier Inc.)
Databáze: MEDLINE
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