Chondroitin sulfate from heads of corb: Recovery, structural analysis and assessment of anticoagulant activity.

Autor: Bougatef H; Laboratory for the Improvement of Plants and Valorization of Agroresources, National School of Engineering of Sfax (ENIS), University of Sfax, Sfax, 3038, Tunisia., Volpi N; Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213/D, 41125, Modena, Italy., Ben Amor I; Sfax Regional Blood Transfusion Center, El-Ain Road Km 0.5, 3003, Sfax, Tunisia., Capitani F; Clinical and Experimental Medicine PhD Program, University of Modena and Reggio Emilia, Modena, Italy., Maccari F; Department of Life Sciences, University of Modena and Reggio Emilia, Via Campi 213/D, 41125, Modena, Italy., Gargouri J; Laboratory of Hematology, Medical Faculty of Sfax. University of Sfax, Magida Boulila Avenue, 3029, Sfax, Tunisia., Sila A; Laboratory for the Improvement of Plants and Valorization of Agroresources, National School of Engineering of Sfax (ENIS), University of Sfax, Sfax, 3038, Tunisia; Department of Life Sciences, Faculty of Sciences of Gafsa, University of Gafsa, 2100, Gafsa, Tunisia., Bougatef A; Laboratory for the Improvement of Plants and Valorization of Agroresources, National School of Engineering of Sfax (ENIS), University of Sfax, Sfax, 3038, Tunisia; High Institute of Biotechnology of Sfax, University of Sfax, Sfax, 3038, Tunisia. Electronic address: ali.bougatef@isbs.usf.tn.
Jazyk: angličtina
Zdroj: Carbohydrate research [Carbohydr Res] 2024 Jul; Vol. 541, pp. 109163. Date of Electronic Publication: 2024 May 23.
DOI: 10.1016/j.carres.2024.109163
Abstrakt: In this study, glycosaminoglycans (GAGs) were extracted from corb (Sciaena umbra) heads and thoroughly examined for their structure. Through cellulose acetate electrophoresis, the GAGs were identified as chondroitin sulfate (CS), with a recovery yield of 10.35 %. The CS exhibited notable characteristics including a high sulfate content (12.4 %) and an average molecular weight of 38.32 kDa. Further analysis via 1 H NMR spectroscopy and SAX-HPLC revealed that the CS primarily consisted of alternating units predominantly composed of monosulfated disaccharides at positions 6 and 4 of GalNAc (52.6 % and 38.8 %, respectively). The ratio of sulfate groups between positions 4 and 6 of GalNAc (4/6 ratio) was approximately 0.74, resulting in an overall charge density of 0.98. Thermal properties of the CS were assessed using techniques such as differential scanning calorimetry and thermogravimetric analysis. Notably, the CS demonstrated concentration-dependent prolongation of activated partial thromboplastin time (aPTT) and thrombin time (TT) while showing no effect on platelet function. At 200 μg/mL, aPTT and TT coagulation times were 1.4 and 3.7 times faster than the control, respectively. These findings suggest that CS derived from corb heads holds promise as an anticoagulant agent for therapy, although further clinical investigations are necessary to validate its efficacy.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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