The Salmonella virulence protein PagN contributes to the advent of a hyper-replicating cytosolic bacterial population.

Autor:	Holbert S; INRAE, Université de Tours, ISP, Nouzilly, France., Barilleau E; INRAE, Université de Tours, ISP, Nouzilly, France., Yan J; IRSD - Institut de Recherche en Santé Digestive, ENVT, INRAE, INSERM, Université́ de Toulouse, UPS, Toulouse, France.; Department of Gastroenterology, The Second Xiangya Hospital of Central South University, China.; Research Center of Digestive Disease, Central South University, China., Trotereau J; INRAE, Université de Tours, ISP, Nouzilly, France., Koczerka M; INRAE, Université de Tours, ISP, Nouzilly, France., Charton M; INRAE, Université de Tours, ISP, Nouzilly, France.; Service biologie vétérinaire et santé animale, Inovalys, Angers, France., Le Vern Y; INRAE, Université de Tours, ISP, Nouzilly, France., Pichon J; INRAE, Université de Tours, ISP, Nouzilly, France., Grassl GA; Institute of Medical Microbiology and Hospital Epidemiology, Hannover Medical School and German Center for Infection Research (DZIF), Hannover, Germany., Velge P; INRAE, Université de Tours, ISP, Nouzilly, France., Wiedemann A; INRAE, Université de Tours, ISP, Nouzilly, France.; IRSD - Institut de Recherche en Santé Digestive, ENVT, INRAE, INSERM, Université́ de Toulouse, UPS, Toulouse, France.
Jazyk:	angličtina
Zdroj:	Virulence [Virulence] 2024 Dec; Vol. 15 (1), pp. 2357670. Date of Electronic Publication: 2024 May 28.
DOI:	10.1080/21505594.2024.2357670
Abstrakt:	Salmonella enterica subspecies enterica serovar Typhimurium is an intracellular pathogen that invades and colonizes the intestinal epithelium. Following bacterial invasion, Salmonella is enclosed within a membrane-bound vacuole known as a Salmonella -containing vacuole (SCV). However, a subset of Salmonella has the capability to prematurely rupture the SCV and escape, resulting in Salmonella hyper-replication within the cytosol of epithelial cells. A recently published RNA-seq study provides an overview of cytosolic and vacuolar upregulated genes and highlights pagN vacuolar upregulation. Here, using transcription kinetics, protein production profile, and immunofluorescence microscopy, we showed that PagN is exclusively produced by Salmonella in SCV. Gentamicin protection and chloroquine resistance assays were performed to demonstrate that deletion of pagN affects Salmonella replication by affecting the cytosolic bacterial population. This study presents the first example of a Salmonella virulence factor expressed within the endocytic compartment, which has a significant impact on the dynamics of Salmonella cytosolic hyper-replication.
Databáze:	MEDLINE
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