The Polysaccharides from Pinellia ternata and Their Derivatives: Preparation, Structure Characteristics, and Activities in Vitro.

Autor: Zhao XL; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, PR China.; Key Laboratory of Drug Screening and Deep Processing for Traditional Chinese and Tibetan Medicine of Gansu Province, Lanzhou, 730050, PR China., Guo ZL; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, PR China., Qi KL; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, PR China., Zhou QS; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, PR China., Liu YM; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, PR China., Xiong LX; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, PR China., Zhang J; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, PR China.; Key Laboratory of Drug Screening and Deep Processing for Traditional Chinese and Tibetan Medicine of Gansu Province, Lanzhou, 730050, PR China., Zhang J; College of Life Science, Northwest Normal University, Lanzhou, 730070, PR China., Zhang WJ; School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou, 730050, PR China.; Key Laboratory of Drug Screening and Deep Processing for Traditional Chinese and Tibetan Medicine of Gansu Province, Lanzhou, 730050, PR China.
Jazyk: angličtina
Zdroj: Chemistry & biodiversity [Chem Biodivers] 2024 Aug; Vol. 21 (8), pp. e202400596. Date of Electronic Publication: 2024 Jul 06.
DOI: 10.1002/cbdv.202400596
Abstrakt: Three polysaccharides, PTC, PTH, and PTB, were extracted from Pinellia ternata using three different extraction conditions: room temperature water, hot water, and 2 % Na 2 CO 3 solution. PTC and PTH were composed of rhamnose, glucose, galactose, mannose, glucuronic acid, galacturonic acid, and arabinose, which combine to form complex structures. PTB was composed solely of glucose and rhamnose. Further analysis indicated that PTC and PTB exhibited triple-helix structures. PTC showed the highest scavenging capacity against DPPH, superoxide anion, and hydroxyl radicals, with half maximal inhibitory concentrations (IC 50 ) of 1004.1, 1584.1, and 1584.1 μg/mL, respectively. Additionally, PTC, PTH, and PTB were subjected to sulfation, phosphorylation, and selenization, resulting in the production of nine derivates. The distinctive absorptive bands of these derivates were determined through infrared spectroscopy. Selenized and sulfated derivates have shown significant antitumor and immunoenhancing properties. Our findings revealed that at 400 μg/mL, the inhibition rate of selenated PTB on HeLa cells was 54.2 % and that on HepG2 cells was 43.1 %. Additionally, selenized PTC displayed significant immunoenhancing activity, with a proliferation rate of 63.7 % at 400 μg/mL in RAW264.7 cells. These results provide valuable evidence supporting the consideration of polysaccharides from Pinellia ternata as a potential candidate for the development of antineoplastic drugs.
(© 2024 Wiley-VHCA AG, Zurich, Switzerland.)
Databáze: MEDLINE
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