The effects of doxapram and its potential interactions with K2P channels in experimental model preparations.

Autor: Elliott ER; Department of Biology, University of Kentucky, Lexington, KY, 40506-0225, USA., Brock KE; Department of Biology, University of Kentucky, Lexington, KY, 40506-0225, USA., Vacassenno RM; Department of Biology, University of Kentucky, Lexington, KY, 40506-0225, USA., Harrison DA; Department of Biology, University of Kentucky, Lexington, KY, 40506-0225, USA., Cooper RL; Department of Biology, University of Kentucky, Lexington, KY, 40506-0225, USA. RLCOOP1@UKY.EDU.
Jazyk: angličtina
Zdroj: Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology [J Comp Physiol A Neuroethol Sens Neural Behav Physiol] 2024 Nov; Vol. 210 (6), pp. 869-884. Date of Electronic Publication: 2024 May 27.
DOI: 10.1007/s00359-024-01705-6
Abstrakt: The channels commonly responsible for maintaining cell resting membrane potentials are referred to as K2P (two-P-domain K + subunit) channels. These K + ion channels generally remain open but can be modulated by their local environment. These channels are classified based on pharmacology, pH sensitivity, mechanical stretch, and ionic permeability. Little is known about the physiological nature of these K2P channels in invertebrates. Acidic conditions depolarize neurons and muscle fibers, which may be caused by K2P channels given that one subtype can be blocked by acidic conditions. Doxapram is used clinically as a respiratory aid known to block acid-sensitive K2P channels; thus, the effects of doxapram on the muscle fibers and synaptic transmission in larval Drosophila and crawfish were monitored. A dose-dependent response was observed via depolarization of the larval Drosophila muscle and an increase in evoked synaptic transmission, but doxapram blocked the production of action potentials in the crawfish motor neuron and had a minor effect on the resting membrane potential of the crawfish muscle. This indicates that the nerve and muscle tissues in larval Drosophila and crawfish likely express different K2P channel subtypes. Since these organisms serve as physiological models for neurobiology and physiology, it would be of interest to further investigate what types of K2P channel are expressed in these tissues. (212 words).
Competing Interests: Declarations Competing interests The authors declare no competing interests.
(© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)
Databáze: MEDLINE
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