Insights into the early-life chemical exposome of Nigerian infants and potential correlations with the developing gut microbiome.

Autor: Oesterle I; University of Vienna, Faculty of Chemistry, Department of Food Chemistry and Toxicology, 1090 Vienna, Austria; Universität Wien, Fakultät für Chemie, Institut für Biophysikalische Chemie, 1090 Wien, Austria(1); University of Vienna, Vienna Doctoral School of Chemistry (DoSChem), 1090 Vienna, Austria., Ayeni KI; University of Vienna, Faculty of Chemistry, Department of Food Chemistry and Toxicology, 1090 Vienna, Austria; Department of Microbiology, Babcock University, Ilishan-Remo, Ogun State, Nigeria., Ezekiel CN; Department of Microbiology, Babcock University, Ilishan-Remo, Ogun State, Nigeria; University of Natural Resources and Life Sciences Vienna (BOKU), Department of Agrobiotechnology (IFA-Tulln), Institute for Bioanalytics and Agro-Metabolomics, Konrad-Lorenz Str. 20, 3430 Tulln, Austria., Berry D; University of Vienna, Division of Microbial Ecology, Department of Microbiology and Ecosystem Science, Centre for Microbiology and Environmental Systems Science, 1030 Vienna, Austria., Rompel A; Universität Wien, Fakultät für Chemie, Institut für Biophysikalische Chemie, 1090 Wien, Austria(1); University of Vienna, Vienna Doctoral School of Chemistry (DoSChem), 1090 Vienna, Austria., Warth B; University of Vienna, Faculty of Chemistry, Department of Food Chemistry and Toxicology, 1090 Vienna, Austria; University of Vienna, Vienna Doctoral School of Chemistry (DoSChem), 1090 Vienna, Austria; Exposome Austria, Research Infrastructure and National EIRENE Node, Austria. Electronic address: benedikt.warth@univie.ac.at.
Jazyk: angličtina
Zdroj: Environment international [Environ Int] 2024 Jun; Vol. 188, pp. 108766. Date of Electronic Publication: 2024 May 22.
DOI: 10.1016/j.envint.2024.108766
Abstrakt: Early-life exposure to natural and synthetic chemicals can impact acute and chronic health conditions. Here, a suspect screening workflow anchored on high-resolution mass spectrometry was applied to elucidate xenobiotics in breast milk and matching stool samples collected from Nigerian mother-infant pairs (n = 11) at three time points. Potential correlations between xenobiotic exposure and the developing gut microbiome, as determined by 16S rRNA gene amplicon sequencing, were subsequently explored. Overall, 12,192 and 16,461 features were acquired in the breast milk and stool samples, respectively. Following quality control and suspect screening, 562 and 864 features remained, respectively, with 149 of these features present in both matrices. Taking advantage of 242 authentic reference standards measured for confirmatory purposes of food bio-actives and toxicants, 34 features in breast milk and 68 features in stool were identified and semi-quantified. Moreover, 51 and 78 features were annotated with spectral library matching, as well as 416 and 652 by in silico fragmentation tools in breast milk and stool, respectively. The analytical workflow proved its versatility to simultaneously determine a diverse panel of chemical classes including mycotoxins, endocrine-disrupting chemicals (EDCs), antibiotics, plasticizers, perfluorinated alkylated substances (PFAS), and pesticides, although it was originally optimized for polyphenols. Spearman rank correlation of the identified features revealed significant correlations between chemicals of the same classification such as polyphenols. One-way ANOVA and differential abundance analysis of the data obtained from stool samples revealed that molecules of plant-based origin elevated as complementary foods were introduced to the infants' diets. Annotated compounds in the stool, such as tricetin, positively correlated with the genus Blautia. Moreover, vulgaxanthin negatively correlated with Escherichia-Shigella. Despite the limited sample size, this exploratory study provides high-quality exposure data of matched biospecimens obtained from mother-infant pairs in sub-Saharan Africa and shows potential correlations between the chemical exposome and the gut microbiome.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024. Published by Elsevier Ltd.)
Databáze: MEDLINE
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