Multiplex imaging reveals spatially resolved DNA-damage response neighborhoods in TP53-mutated myelodysplastic neoplasms.

Autor: Yeung T; Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY, USA., Zhang Y; Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY, USA., Kennedy B; Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY, USA., Walsh C; Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY, USA., Love T; Department of Biostatistics and Computational Biology, University of Rochester, Rochester, NY, USA., Xia D; Department of Pathology, University Health Network, Toronto, ON, Canada.; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada., Bhattacharya A; Department of Computer Science, University of Toronto, ON, Canada., Krishnan RG; Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto, ON, Canada.; Department of Computer Science, University of Toronto, ON, Canada., Head D; Department of Pathology, Microbiology and Immunology, Vanderbilt University Medical Center, Nashville, TN, USA., Burack R; Pathology and Laboratory Medicine, University of Rochester Medical Center, Rochester, NY, USA.
Jazyk: angličtina
Zdroj: The Journal of pathology [J Pathol] 2024 Jul; Vol. 263 (3), pp. 386-395. Date of Electronic Publication: 2024 May 27.
DOI: 10.1002/path.6292
Abstrakt: While increased DNA damage is a well-described feature of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML), it is unclear whether all lineages and all regions of the marrow are homogeneously affected. In this study, we performed immunohistochemistry on formalin-fixed, paraffin-embedded whole-section bone marrow biopsies using a well-established antibody to detect pH2A.X (phosphorylated histone variant H2A.X) that recognizes DNA double-strand breaks. Focusing on TP53-mutated and complex karyotype MDS/AML, we find a greater pH2A.X+ DNA damage burden compared to TP53 wild-type neoplastic cases and non-neoplastic controls. To understand how double-strand breaks vary between lineages and spatially in TP53-mutated specimens, we applied a low-multiplex immunofluorescence staining and spatial analysis protocol to visualize pH2A.X+ cells with p53 protein staining and lineage markers. pH2A.X marked predominantly mid- to late-stage erythroids, whereas early erythroids and CD34+ blasts were relatively spared. In a prototypical example, these pH2A.X+ erythroids were organized locally as distinct colonies, and each colony displayed pH2A.X+ puncta at a synchronous level. This highly coordinated immunophenotypic expression was also seen for p53 protein staining and among presumed early myeloid colonies. Neighborhood clustering analysis showed distinct marrow regions differentially enriched in pH2A.X+/p53+ erythroid or myeloid colonies, indicating spatial heterogeneity of DNA-damage response and p53 protein expression. The lineage and architectural context within which DNA damage phenotype and oncogenic protein are expressed is relevant to current therapeutic developments that leverage macrophage phagocytosis to remove leukemic cells in part due to irreparable DNA damage. © 2024 The Pathological Society of Great Britain and Ireland.
(© 2024 The Pathological Society of Great Britain and Ireland.)
Databáze: MEDLINE
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