Influence of previous plane of nutrition on molecular mechanisms regulating the expression of urea and water metabolism related genes in the rumen and kidney of finishing crossbred Angus steers.
| Autor: | Moreira da Silva AE; College of Agriculture, Biotechnology & Natural Resources, University of Nevada, Reno, Reno, NV 89503, USA., Franco AM; College of Agriculture, Biotechnology & Natural Resources, University of Nevada, Reno, Reno, NV 89503, USA., Ferguson BS; College of Agriculture, Biotechnology & Natural Resources, University of Nevada, Reno, Reno, NV 89503, USA., Fonseca MA; College of Agriculture, Biotechnology & Natural Resources, University of Nevada, Reno, Reno, NV 89503, USA.; Department of Animal and Range Sciences, Clayton Livestock Research Center, New Mexico State University, Clayton, NM 88415, USA. |
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| Jazyk: | angličtina |
| Zdroj: | Animal nutrition (Zhongguo xu mu shou yi xue hui) [Anim Nutr] 2024 Mar 16; Vol. 17, pp. 232-243. Date of Electronic Publication: 2024 Mar 16 (Print Publication: 2024). |
| DOI: | 10.1016/j.aninu.2023.12.011 |
| Abstrakt: | This study aimed to understand how molecular mechanisms controlling water and urea metabolism at the finishing phase can be affected by previous plane of nutrition of crossbred Angus beef steers. Twenty-four ( n = 24) animals were randomly distributed into either a moderate (MP) or high plane of nutrition during the background phase for 85 d. Animals were then blocked by their previous plane and were moved onto a 105-d finishing phase in a 2 × 2 factorial arrangement. The forage-finished group received only high-quality alfalfa hay, whereas the grain-fed group received a high grain diet (80% whole corn and 20% alfalfa hay). By the end of the finishing phase, animals were harvested and tissue samples from the rumen and kidney were collected. Changes in gene expression of aquaporins ( AQP )- 2 , - 3 , - 4 , - 7 , ATP1A1 , ATP1B1 , SGK1 , CLIC1 (kidney and rumen), UT-A1 (kidney only) and UT-B (rumen only), were assayed via real-time qPCR; 18S rRNA was used as an endogenous control. One-way ANOVA followed by Tukey's post hoc analysis was conducted. When animals were from MP, forage-finishing increased the relative abundance of AQP3 ( P ≤ 0.05), AQP7 ( P ≤ 0.05), ATP1B1 ( P ≤ 0.05), and SGK1 ( P ≤ 0.05) in the kidney when compared to grain-fed animals. In the rumen, for the MP group, AQP7 was differentially expressed in both treatments at the finishing phase ( P ≤ 0.01), with forage-finished steers having the highest expression of AQP7 . For the MP group, UT-B had a tendency of presenting a higher expression on grain-fed animals ( P = 0.075). Overall, these results suggest that previous plane can impact expression of genes associated with water and urea metabolism during the finishing phase, namely AQP3 , AQP7 , ATP1B1 , and SGK1 in the kidney, and AQP7 and UT-B in the rumen. The greatest impact observed on gene expression changes of investigated genes at the finishing phase was reflective of animals backgrounded on the moderate previous plane. Competing Interests: We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, and there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the content of this paper. (© 2024 The Authors.) |
| Databáze: | MEDLINE |
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