Aberrant microbiomes are associated with increased antibiotic resistance gene load in hybrid mice.

Autor:	Jarquín-Díaz VH; Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC). Robert-Rössle-Str. 10, 13125 Berlin, Germany.; Leibniz Institute for Zoo and Wildlife Research (IZW), Alfred-Kowalke-Straße 17, 10315, Berlin, Germany.; Experimental and Clinical Research Center, a cooperation between the Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association and the Charité-Universitätsmedizin, Berlin, Germany.; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Experimental and Clinical Research Center, Lindenberger Weg 80, 13125, Berlin, Germany.; Department of Molecular Parasitology, Institute for Biology, Humboldt University Berlin (HU). Philippstr. 13, Haus 14, 10115, Berlin, Germany., Ferreira SCM; Department of Molecular Parasitology, Institute for Biology, Humboldt University Berlin (HU). Philippstr. 13, Haus 14, 10115, Berlin, Germany.; Division of Computational Systems Biology, Center for Microbiology and Ecological System Science, University of Vienna, Djerassipl. 1, 1030, Vienna, Austria., Balard A; Leibniz Institute for Zoo and Wildlife Research (IZW), Alfred-Kowalke-Straße 17, 10315, Berlin, Germany.; Department of Molecular Parasitology, Institute for Biology, Humboldt University Berlin (HU). Philippstr. 13, Haus 14, 10115, Berlin, Germany., Ďureje Ľ; Research Facility Studenec, Institute of Vertebrate Biology, Czech Academy of Sciences, Květná 8, 60365, Brno, Czech Republic., Macholán M; Laboratory of Mammalian Evolutionary Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, Veveri 97, 60200, Brno, Czech Republic., Piálek J; Research Facility Studenec, Institute of Vertebrate Biology, Czech Academy of Sciences, Květná 8, 60365, Brno, Czech Republic., Bengtsson-Palme J; Division of Systems and Synthetic Biology, Department of Life Sciences, SciLifeLab, Chalmers University of Technology, Kemivägen 10, SE-412 96, Gothenburg, Sweden.; Department of Infectious Diseases, Institute of Biomedicine, The Sahlgrenska Academy, University of Gothenburg, SE-413 46, Gothenburg, Sweden.; Centre for Antibiotic Resistance Research (CARe) in Gothenburg, Sweden., Kramer-Schadt S; Leibniz Institute for Zoo and Wildlife Research (IZW), Alfred-Kowalke-Straße 17, 10315, Berlin, Germany.; Institute of Ecology, Technische Universität Berlin, Rothenburgstr. 12, 12165, Berlin, Germany., Forslund-Startceva SK; Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association (MDC). Robert-Rössle-Str. 10, 13125 Berlin, Germany.; Experimental and Clinical Research Center, a cooperation between the Max-Delbrück-Center for Molecular Medicine in the Helmholtz Association and the Charité-Universitätsmedizin, Berlin, Germany.; Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Experimental and Clinical Research Center, Lindenberger Weg 80, 13125, Berlin, Germany.; German Centre for Cardiovascular Research (DZHK), Partner Site Berlin, Berlin, Germany., Heitlinger E; Leibniz Institute for Zoo and Wildlife Research (IZW), Alfred-Kowalke-Straße 17, 10315, Berlin, Germany.; Department of Molecular Parasitology, Institute for Biology, Humboldt University Berlin (HU). Philippstr. 13, Haus 14, 10115, Berlin, Germany.
Jazyk:	angličtina
Zdroj:	ISME communications [ISME Commun] 2024 Apr 15; Vol. 4 (1), pp. ycae053. Date of Electronic Publication: 2024 Apr 15 (Print Publication: 2024).
DOI:	10.1093/ismeco/ycae053
Abstrakt:	Antibiotic resistance is a priority public health problem resulting from eco-evolutionary dynamics within microbial communities and their interaction at a mammalian host interface or geographical scale. The links between mammalian host genetics, bacterial gut community, and antimicrobial resistance gene (ARG) content must be better understood in natural populations inhabiting heterogeneous environments. Hybridization, the interbreeding of genetically divergent populations, influences different components of the gut microbial communities. However, its impact on bacterial traits such as antibiotic resistance is unknown. Here, we present that hybridization might shape bacterial communities and ARG occurrence. We used amplicon sequencing to study the gut microbiome and to predict ARG composition in natural populations of house mice ( Mus musculus ). We compared gastrointestinal bacterial and ARG diversity, composition, and abundance across a gradient of pure and hybrid genotypes in the European House Mouse Hybrid Zone. We observed an increased overall predicted richness of ARG in hybrid mice. We found bacteria-ARG interactions by their co-abundance and detected phenotypes of extreme abundances in hybrid mice at the level of specific bacterial taxa and ARGs, mainly multidrug resistance genes. Our work suggests that mammalian host genetic variation impacts the gut microbiome and chromosomal ARGs. However, it raises further questions on how the mammalian host genetics impact ARGs via microbiome dynamics or environmental covariates. Competing Interests: The authors declare there are any competing financial interests concerning the work. (© The Author(s) 2024. Published by Oxford University Press on behalf of the International Society for Microbial Ecology.)
Databáze:	MEDLINE
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