High-yield fabrication of monodisperse multilayer nanofibrous microparticles for advanced oral drug delivery applications.

Autor:	Ajalloueian F; Department of Health Technology, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark.; The Danish National Research Foundation and Villum Foundation's Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Ørsted Plads, 2800, Kgs. Lyngby, Denmark., Eklund Thamdrup LH; Department of Health Technology, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark.; The Danish National Research Foundation and Villum Foundation's Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Ørsted Plads, 2800, Kgs. Lyngby, Denmark., Mazzoni C; Department of Health Technology, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark.; The Danish National Research Foundation and Villum Foundation's Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Ørsted Plads, 2800, Kgs. Lyngby, Denmark., Petersen RS; National Centre for Nano Fabrication and Characterization, DTU Nanolab, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark., Keller SS; National Centre for Nano Fabrication and Characterization, DTU Nanolab, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark., Boisen A; Department of Health Technology, Technical University of Denmark, 2800, Kgs. Lyngby, Denmark.; The Danish National Research Foundation and Villum Foundation's Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics (IDUN), Department of Health Technology, Technical University of Denmark, Ørsted Plads, 2800, Kgs. Lyngby, Denmark.
Jazyk:	angličtina
Zdroj:	Heliyon [Heliyon] 2024 May 08; Vol. 10 (10), pp. e30844. Date of Electronic Publication: 2024 May 08 (Print Publication: 2024).
DOI:	10.1016/j.heliyon.2024.e30844
Abstrakt:	Recent advances in the use of nano- and microparticles in drug delivery, cell therapy, and tissue engineering have led to increasing attention towards nanostructured microparticulate formulations for maximum benefit from both nano- and micron sized features. Scalable manufacturing of monodisperse nanostructured microparticles with tunable size, shape, content, and release rate remains a big challenge. Current technology, mainly comprises complex multi-step chemical procedures with limited control over these aspects. Here, we demonstrate a novel technique for high-yield fabrication of monodisperse monolayer and multilayer nanofibrous microparticles (MoNami and MuNaMi respectively). The fabrication procedure includes sequential electrospinning followed by micro-cutting at room temperature and transfer of particles for collection. The big advantage of the introduced technique is the potential to apply several polymer-drug combinations forming multilayer microparticles enjoying extracellular matrix (ECM)-mimicking architecture with tunable release profile. We demonstrate the fabrication and study the factors affecting the final three-dimensional structure. A model drug is encapsulated into a three-layer sheet (PLGA-pullulan-PLGA), and we demonstrate how the release profile changes from burst to sustain by simply cutting particles out of the electrospun sheet. We believe our fabrication method offers a unique and facile platform for realizing advanced microparticles for oral drug delivery applications. Competing Interests: The authors declare no competing interests. However, we would like to disclose that the Technical University of Denmark has filed a patent (PCT/EP2024/055263) entitled “Monodisperse nanofibrous microparticles for delivery of one or more active substances” where the inventors are Ajalloueian F., Thamdrup L.H.E. and Boisen A. (© 2024 The Authors.)
Databáze:	MEDLINE
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