Effects of dietary nitrogen and/or phosphorus reduction on mineral homeostasis and regulatory mechanisms in young goats.
| Autor: | Zillinger LS; Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany., Hustedt K; Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany., Schnepel N; Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany., Hirche F; Institute of Agricultural and Nutritional Science, Martin Luther University Halle-Wittenberg, Halle, Germany., Schmicke M; Clinic for Cattle, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany., Stangl GI; Institute of Agricultural and Nutritional Science, Martin Luther University Halle-Wittenberg, Halle, Germany., Muscher-Banse AS; Institute for Physiology and Cell Biology, University of Veterinary Medicine Hannover, Foundation, Hannover, Germany. |
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| Jazyk: | angličtina |
| Zdroj: | Frontiers in veterinary science [Front Vet Sci] 2024 May 10; Vol. 11, pp. 1375329. Date of Electronic Publication: 2024 May 10 (Print Publication: 2024). |
| DOI: | 10.3389/fvets.2024.1375329 |
| Abstrakt: | Introduction: The reduction of nitrogen (N) and phosphorus (P) in ruminant feed is desirable due to costs and negative environmental impact. Ruminants are able to utilize N and P through endogenous recycling, particularly in times of scarcity. When N and/or P were reduced, changes in mineral homeostasis associated with modulation of renal calcitriol metabolism occurred. The aim of this study was to investigate the potential effects of dietary N- and/or P-reduction on the regulatory mechanisms of mineral transport in the kidney and its hormonal regulation in young goats. Results: During N-reduction, calcium (Ca) and magnesium (Mg) concentrations in blood decreased, accompanied by a lower protein expression of cytochrome P450 family 27 subfamily B member 1 (CYP27B1) ( p = 0.016). The P-reduced fed goats had low blood phosphate concentrations with simultaneously high Ca and Mg levels. The insulin-like growth factor 1 concentrations decreased significantly with P-reduction. Furthermore, gene expression of CYP27B1 ( p < 0.001) and both gene ( p = 0.025) and protein ( p = 0.016) expression of the fibroblast growth factor receptor 1c isoform in the kidney were also significantly reduced during a P-reduced diet. ERK1/2 activation exhibited a trend toward reduction in P-reduced animals. Interestingly, calcitriol concentrations remained unaffected by either restriction individually, but interacted significantly with N and P ( p = 0.014). Additionally, fibroblast growth factor 23 mRNA expression in bone decreased significantly with P-restriction ( p < 0.001). Discussion: These results shed light on the complex metabolic and regulatory responses of mineral transport of young goats to dietary N and P restriction. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2024 Zillinger, Hustedt, Schnepel, Hirche, Schmicke, Stangl and Muscher-Banse.) |
| Databáze: | MEDLINE |
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