Characterization of chitosan- and β-cyclodextrin-modified forms of magnesium-doped hydroxyapatites as enhanced carriers for levofloxacin: loading, release, and anti-inflammatory properties.

Autor: Bin Jumah MN; Biology Department, College of Science, Princess Nourah Bint Abdulrahman University Riyadh Saudi Arabia., Al Othman SI; Biology Department, College of Science, Princess Nourah Bint Abdulrahman University Riyadh Saudi Arabia., Alomari AA; Biology Department, College of Science, Princess Nourah Bint Abdulrahman University Riyadh Saudi Arabia., Allam AA; Zoology Department, Faculty of Science, Beni-Suef University Beni-Suef Egypt.; Department of Biology, College of Science, Imam Mohammad Ibn Saud Islamic University (IMSIU) Riyadh 11623 Kingdom of Saudi Arabia., Abukhadra MR; Geology Department, Faculty of Science, Beni-Suef University Beni-Suef 65211 Egypt Abukhadra89@Science.bsu.edu.eg +2001288447189.; Materials Technologies and Their Applications Lab, Geology Department, Faculty of Science, Beni-Suef University Beni-Suef City Egypt.
Jazyk: angličtina
Zdroj: RSC advances [RSC Adv] 2024 May 24; Vol. 14 (24), pp. 16991-17007. Date of Electronic Publication: 2024 May 24 (Print Publication: 2024).
DOI: 10.1039/d4ra02144d
Abstrakt: An advanced form of magnesium-rich hydroxyapatite (Mg·HAP) was modified with two types of biopolymers, namely chitosan (CH/Mg·HAP) and β-cyclodextrin (CD/Mg·HAP), producing two types of bio-composites. The synthesized materials were developed as enhanced carriers for levofloxacin to control its loading, release, and anti-inflammatory properties. The polymeric modification significantly improved the loading efficiency to 281.4 mg g -1 for CH/Mg·HAP and 332.4 mg g -1 for CD/Mg·HAP compared with 218.3 mg g -1 for Mg·HAP. The loading behaviors were determined using conventional kinetic and isotherm models and mathematical parameters of new equilibrium models (the monolayer model of one energy). The estimated density of effective loading sites ( N m (LVX) = 88.03 mg g -1 (Mg·HAP), 115.8 mg g -1 (CH/Mg·HAP), and 138.5 mg g -1 (CD/Mg·HAP)) illustrates the markedly higher loading performance of the modified forms of Mg·HAP. Moreover, the loading energies (<40 kJ mol -1 ) in conjunction with the capacity of each loading site ( n > 1) and Gaussian energies (<8 kJ mol -1 ) signify the physical trapping of LVX molecules in vertical orientation. The addressed materials validate prolonged and continuous release behaviors. These behaviors accelerated after the modification procedures, as the complete release was identified after 160 h (CH/Mg·HAP) and 200 h (CD/Mg·HAP). The releasing behaviors are regulated by both diffusion and erosion mechanisms, according to the kinetic investigations and diffusion exponent analysis (>0.45). The entrapping of LVX into Mg·HAP induces its anti-inflammatory properties against the generation of cytokines (IL-6 and IL-8) in human bronchial epithelia cells (NL20), and this effect displays further enhancement after the integration of chitosan and β-cyclodextrin.
Competing Interests: There are no conflicts to declare.
(This journal is © The Royal Society of Chemistry.)
Databáze: MEDLINE
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