Overexpression of stress granule protein TZF1 enhances salt stress tolerance by targeting ACA11 mRNA for degradation in Arabidopsis.

Autor:	He SL; Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH, United States.; Center for Applied Plant Sciences, The Ohio State University, Columbus, OH, United States.; Center for RNA Biology, The Ohio State University, Columbus, OH, United States., Li B; Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and University of Chinese Academy of Sciences, Beijing, China.; Academician Workstation of Agricultural High-Tech Industrial Area of the Yellow River Delta, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Shandong, China., Zahurancik WJ; Center for RNA Biology, The Ohio State University, Columbus, OH, United States.; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, United States., Arthur HC; Center for RNA Biology, The Ohio State University, Columbus, OH, United States.; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, United States., Sidharthan V; Center for RNA Biology, The Ohio State University, Columbus, OH, United States.; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, United States., Gopalan V; Center for RNA Biology, The Ohio State University, Columbus, OH, United States.; Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH, United States., Wang L; Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, and University of Chinese Academy of Sciences, Beijing, China.; Academician Workstation of Agricultural High-Tech Industrial Area of the Yellow River Delta, National Center of Technology Innovation for Comprehensive Utilization of Saline-Alkali Land, Shandong, China., Jang JC; Department of Horticulture and Crop Science, The Ohio State University, Columbus, OH, United States.; Center for Applied Plant Sciences, The Ohio State University, Columbus, OH, United States.; Center for RNA Biology, The Ohio State University, Columbus, OH, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in plant science [Front Plant Sci] 2024 May 08; Vol. 15, pp. 1375478. Date of Electronic Publication: 2024 May 08 (Print Publication: 2024).
DOI:	10.3389/fpls.2024.1375478
Abstrakt:	Tandem CCCH zinc finger (TZF) proteins play diverse roles in plant growth and stress response. Although as many as 11 TZF proteins have been identified in Arabidopsis , little is known about the mechanism by which TZF proteins select and regulate the target mRNAs. Here, we report that Arabidopsis TZF1 is a bona-fide stress granule protein. Ectopic expression of TZF1 ( TZF1 OE ), but not an mRNA binding-defective mutant ( TZF1 H186Y OE ), enhances salt stress tolerance in Arabidopsis . RNA-seq analyses of NaCl-treated plants revealed that the down-regulated genes in TZF1 OE plants are enriched for functions in salt and oxidative stress responses. Because many of these down-regulated mRNAs contain AU- and/or U-rich elements (AREs and/or UREs) in their 3'-UTRs, we hypothesized that TZF1-ARE/URE interaction might contribute to the observed gene expression changes. Results from RNA immunoprecipitation-quantitative PCR analysis, gel-shift, and mRNA half-life assays indicate that TZF1 binds and triggers degradation of the autoinhibited Ca 2+ -ATPase 11 ( ACA11 ) mRNA, which encodes a tonoplast-localized calcium pump that extrudes calcium and dampens signal transduction pathways necessary for salt stress tolerance. Furthermore, this salt stress-tolerance phenotype was recapitulated in aca11 null mutants. Collectively, our findings demonstrate that TZF1 binds and initiates degradation of specific mRNAs to enhance salt stress tolerance. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision. (Copyright © 2024 He, Li, Zahurancik, Arthur, Sidharthan, Gopalan, Wang and Jang.)
Databáze:	MEDLINE
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