Methods to Utilize Pulse Wave Velocity to Measure Alterations in Cerebral and Cardiovascular Parameters.

Autor:	Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Shao B; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Crabtree A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Garza-Lopez E; Department of Internal Medicine, University of Iowa, Iowa City, IA, 52242, USA., Scudese E; Laboratory of Biosciences of Human Motricity (LABIMH) of the Federal University of State of Rio de Janeiro (UNIRIO), Rio de Janeiro, Brazil; Sport Sciences and Exercise Laboratory (LaCEE), Catholic University of Petrópolis (UCP), Brazil., Wanjalla CN; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Kirabo A; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Albritton CF; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.; School of Graduate Studies, Meharry Medical College, Nashville, TN., Jamison S; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA.; School of Graduate Studies, Meharry Medical College, Nashville, TN., Demirci M; Department of Medicine, Vanderbilt University Medical Center, Nashville, TN, 37232, USA., Murray SA; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, 15260, USA., Cooper AT; Department of Cell Biology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, 15260, USA., Taffet GE; Department of Medicine, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA., Hinton AO Jr; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, 37232, USA., Reddy AK; Department of Medicine, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Jazyk:	angličtina
Zdroj:	BioRxiv : the preprint server for biology [bioRxiv] 2024 May 15. Date of Electronic Publication: 2024 May 15.
DOI:	10.1101/2023.06.22.546154
Abstrakt:	Alzheimer's Disease (AD) is a global health issue, affecting over 6 million in the United States, with that number expected to increase as the aging population grows. As a neurodegenerative disorder that affects memory and cognitive functions, it is well established that AD is associated with cardiovascular risk factors beyond only cerebral decline. However, the study of cerebrovascular techniques for AD is still evolving. Here, we provide reproducible methods to measure impedance-based pulse wave velocity (PWV), a marker of arterial stiffness, in the systemic vascular (aortic PWV) and in the cerebral vascular (cerebral PWV) systems. Using aortic impedance and this relatively novel technique of cerebral impedance to comprehensively describe the systemic vascular and the cerebral vascular systems, we examined the sex-dependent differences in 5x transgenic mice (5XFAD) with AD under normal and high-fat diet, and in wild-type mice under a normal diet. Additionally, we validated our method for measuring cerebrovascular impedance in a model of induced stress in 5XFAD. Together, our results show that sex and diet differences in wildtype and 5XFAD mice account for very minimal differences in cerebral impedance. Interestingly, 5XFAD, and not wildtype, male mice on a chow diet show higher cerebral impedance, suggesting pathological differences. Opposingly, when we subjected 5XFAD mice to stress, we found that females showed elevated cerebral impedance. Using this validated method of measuring impedance-based aortic and cerebral PWV, future research may explore the effects of modifying factors including age, chronic diet, and acute stress, which may mediate cardiovascular risk in AD. Competing Interests: Disclosures Dr. Reddy is a collaborator and consultant with Indus Instruments, Webster, TX. All other authors have no competing interests.
Databáze:	MEDLINE
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