Organic acids mitigate Streptococcus agalactiae virulence in Tilapia fish gut primary cells and in a gut infection model.

Autor: Liliana PC; Faculty of Bioengineering of Animal Resources, University of Life Sciences King Mihai I from Timisoara, Timisoara, 300645, Romania., Dumitrescu G; Faculty of Bioengineering of Animal Resources, University of Life Sciences King Mihai I from Timisoara, Timisoara, 300645, Romania., McCleery D; Bacteriology Branch, Veterinary Sciences Division, Agri-Food and Biosciences Institute, Northern Ireland, Belfast, BT4 3SD, UK., Pet I; Faculty of Bioengineering of Animal Resources, University of Life Sciences King Mihai I from Timisoara, Timisoara, 300645, Romania., Iancu T; Faculty of Management and Rural Development, University of Life Sciences King Mihai I from Timisoara, Timisoara, 300645, Romania., Stef L; Faculty of Bioengineering of Animal Resources, University of Life Sciences King Mihai I from Timisoara, Timisoara, 300645, Romania., Corcionivoschi N; Faculty of Bioengineering of Animal Resources, University of Life Sciences King Mihai I from Timisoara, Timisoara, 300645, Romania. Nicolae.corcionivoschi@afbini.gov.uk.; Bacteriology Branch, Veterinary Sciences Division, Agri-Food and Biosciences Institute, Northern Ireland, Belfast, BT4 3SD, UK. Nicolae.corcionivoschi@afbini.gov.uk.; Academy of Romanian Scientists, Ilfov Street, No. 3, Bucharest, 050044, Romania. Nicolae.corcionivoschi@afbini.gov.uk., Balta I; Faculty of Bioengineering of Animal Resources, University of Life Sciences King Mihai I from Timisoara, Timisoara, 300645, Romania. balta.igori@usvt.ro.
Jazyk: angličtina
Zdroj: Irish veterinary journal [Ir Vet J] 2024 May 27; Vol. 77 (1), pp. 10. Date of Electronic Publication: 2024 May 27.
DOI: 10.1186/s13620-024-00272-1
Abstrakt: Background: Streptococcus agalactiae, a Gram-positive bacterium, has emerged as an important pathogen for the aquaculture industry worldwide, due to its increased induced mortality rates in cultured fish. Developing interventions to cure or prevent infections based on natural alternatives to antibiotics has become a priority, however, given the absence of scientific evidence regarding their mode of action progress has been slow.
Methods: In this study we aimed to investigate the effect of a mixture of organic acids (natural antimicrobials), AuraAqua (Aq), on the virulence of S. agalactiae using Tilapia gut primary epithelial cells and an in vitro Tilapia gut culture model. Our results show that Aq was able to reduce significantly, in vitro, the S. agalactiae levels of infection in Tilapia gut primary epithelial cells (TGP) when the MIC concentration of 0.125% was tested.
Results and Discussion: At bacterial level, Aq was able to downregulate bacterial capsule polysaccharide (CPS) gene expression, capC, resulting in a significant decrease in bacterial surface capsule production. The decrease in CPS production was also associated with a reduction in the pro-inflammatory IFNγ, IL1β, TNFα, SOD and CAT gene expression and H 2 O 2 production in the presence of 0.125% Aq (P < 0.0001). The antimicrobial mixture also reduced the levels of S. agalactiae infection in an in vitro gut culture model and significantly reduced the IFNγ, IL1β, TNFα, SOD, CAT gene expression and H 2 O 2 production in infected tissue. Moreover, genes involved in Tilapia resistance to S. agalactiae induced disease, MCP-8 and Duo-1, were also downregulated by Aq, as a consequence of reduced bacterial levels of infection.
Conclusion: Conclusively, our study shows that mixtures of organic acids can be considered as potential alternative treatments to antibiotics and prevent S. agalactiae infection and inflammation in the Tilapia fish digestive tract.
(© 2024. The Author(s).)
Databáze: MEDLINE
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