Volatile organic compounds produced during postmortem processes can be linked via chromatographic profiles to individual postmortem bacterial species.

Autor: Furuta K; Laboratory of Forensic and Bioanalytical Chemistry, School of Natural Sciences and Mathematics, Chaminade University of Honolulu, United States., Byrne J; Laboratory of Forensic and Bioanalytical Chemistry, School of Natural Sciences and Mathematics, Chaminade University of Honolulu, United States., Luat K; School of Natural Sciences and Mathematics, Chaminade University of Honolulu, United States., Cheung C; Laboratory of Forensic and Bioanalytical Chemistry, School of Natural Sciences and Mathematics, Chaminade University of Honolulu, United States., Carter DO; Laboratory of Forensic Taphonomy, School of Natural Sciences and Mathematics, Chaminade University of Honolulu, United States., Tipton L; School of Natural Sciences and Mathematics, Chaminade University of Honolulu, United States; Departments of Biology and Mathematics & Statistics, James Madison University, United States., Perrault Uptmor KA; Laboratory of Forensic and Bioanalytical Chemistry, School of Natural Sciences and Mathematics, Chaminade University of Honolulu, United States; Nontargeted Separations Laboratory, Department of Chemistry, William & Mary, United States. Electronic address: kaperrault@wm.edu.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2024 Aug 02; Vol. 1728, pp. 465017. Date of Electronic Publication: 2024 May 22.
DOI: 10.1016/j.chroma.2024.465017
Abstrakt: Decomposition odor is produced during postmortem mammalian tissue breakdown by bacteria, insects, and intrinsic chemical processes. Past research has not thoroughly investigated which volatile organic compounds (VOCs) can be linked directly to individual bacterial species on decomposing remains. The purpose of this study was to profile the VOCs produced over time by individual species of bacteria using comprehensive two-dimensional gas chromatography (GC×GC) to expand our foundational knowledge of what each bacterial species contributes to decomposition odor. Five different species of bacteria (Bacillus subtilis, Ignatzschineria indica, Ignatzschineria ureiclastica, Curtobacterium luteum, and Vagococcus lutrae) were cultured on standard nutrient agar individually and monitored daily using solid phase microextraction arrow (SPME Arrow) and GC×GC in combination with quadrupole mass spectrometry (qMS) and flame ionization detection (FID). The GC×GC-qMS/FID approach was used to generate rich VOC profiles that represented the bacterial species' metabolic VOC production longitudinally. The data obtained from the chromatographic output was used to compare with a prior study using one-dimensional GC-qMS, and also between each of the five species to investigate the extent of overlap between species. No single VOC could be found in all five bacterial species investigated, and there was little overlap in the profile between species. To further visualize these differences, chromatographic peak data was investigated using two different ordination strategies, principal component analysis (PCA) and principal coordinate analysis (PCoA). The two ordination strategies were compared with each other using a Procrustes analysis. This was performed to understand differences in ordination strategies between the separation science community and chemical ecological community. Overall, ordination strategies were found to produce similar results, as evidenced by the correlation of PCA and PCoA in the Procrustes analysis. All analysis strategies yielded distinct VOC profiles for each species. Further study of additional species will support understanding of the holistic view of decomposition odor from a chemical ecology perspective, and further support our understanding of the production of decomposition odor that culminates from such a complex environment.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Katelynn A. Perrault Uptmor reports financial support was provided by National Science Foundation. Katelynn A. Perrault Uptmor reports equipment, drugs, or supplies was provided by Restek Corp. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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