Bifunctional Tb(III)-modified Ce-MOF nanoprobe for colorimetric and fluorescence sensing of α-glucosidase activity.

Autor: Xiao Y; School of Chemistry and Chemical Engineering, Nanchang University, Nanchang, 330031, China., Huang P; School of Chemistry and Chemical Engineering, Nanchang University, Nanchang, 330031, China. Electronic address: pchuang@ncu.edu.cn., Wu FY; School of Chemistry and Chemical Engineering, Nanchang University, Nanchang, 330031, China. Electronic address: fywu@ncu.edu.cn.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2024 Aug 15; Vol. 276, pp. 126304. Date of Electronic Publication: 2024 May 22.
DOI: 10.1016/j.talanta.2024.126304
Abstrakt: α-Glucosidase, which directly involves in the metabolism of starch and glycogen and causes an increase in blood sugar level, is the major target enzyme for the precaution and therapy of type II diabetes. Based on the previous work, we adopted a post-synthetic modification method to encapsulate Tb 3+ into Ce-MOF nanozyme which owned mixed valence states. Tb@Ce-MOF displayed induced luminescence characteristic and exceptional oxidase-like activity that could oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue ox-TMB. α-Glucosidase can hydrolyze the substrate l-ascorbic acid-2-O-α-d-glucopyranosyl (AAG) to generate ascorbic acid (AA), which could increase the Ce 3+ /Ce 4+ redox valence mode in Tb@Ce-MOF, leading to the inhibition of the allochroic reaction of TMB and the decreased absorption of ox-TMB at 652 nm. The energy transfer (EnT) process from Ce 3+ to Tb 3+ will enhance due to the increased Ce 3+ /Ce 4+ mode in Tb@Ce-MOF, which will result in an enhanced fluorescence signal of Tb@Ce-MOF at 550 nm. But the addition of inhibitor acarbose will inhibit the above process. We have constructed a dual-mode detection platform of α-glucosidase and its inhibitor via colorimetric and fluorometric method. The linear range of α-glucosidase were 0.01-0.5 U/mL (colorimetric mode) and 0.8-1.5 U/mL (fluorometric mode), respectively, with a detection limit as low as 0.0018 U/mL. Furthermore, our approach was also successfully employed to the analysis of α-glucosidase in serum samples.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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