Osteoblast responsive biosilica-enriched gelatin microfibrillar microenvironments.

Autor: Olăreț E; Advanced Polymer Materials Group, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania., Dinescu S; Department of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, Romania; Research Institute of the University of Bucharest (ICUB), 050663 Bucharest, Romania., Dobranici AE; Department of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, Romania., Ginghină RE; Research and Innovation Center for CBRN Defense and Ecology, 041327 Bucharest, Romania., Voicu G; Department of Science and Engineering of Oxide Materials and Nanomaterials, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania; Faculty of Medical Engineering, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania., Mihăilescu M; Faculty of Medical Engineering, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania; Faculty of Applied Sciences, National University of Science and Technology Politehnica Bucharest, 060042 Bucharest, Romania., Curti F; Advanced Polymer Materials Group, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania; Zentiva SA, 50, Theodor Pallady, 032266 Bucharest, Romania., Banciu DD; Faculty of Medical Engineering, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania., Sava B; attocube AG, Haar, Germany., Amarie S; attocube AG, Haar, Germany., Lungu A; Advanced Polymer Materials Group, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania., Stancu IC; Advanced Polymer Materials Group, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania; Faculty of Medical Engineering, National University of Science and Technology Politehnica Bucharest, 011061 Bucharest, Romania. Electronic address: izabela.stancu@upb.ro., Mastalier BSM; University of Medicine and Pharmacy Carol Davila, Bucharest, Romania; Department of General Surgery, Colentina Clinical Hospital, 072202 Bucharest, Romania.
Jazyk: angličtina
Zdroj: Biomaterials advances [Biomater Adv] 2024 Jul; Vol. 161, pp. 213894. Date of Electronic Publication: 2024 May 17.
DOI: 10.1016/j.bioadv.2024.213894
Abstrakt: Engineering of scaffolds for bone regeneration is often inspired by the native extracellular matrix mimicking its composite fibrous structure. In the present study, we used low loadings of diatomite earth (DE) biosilica to improve the bone regeneration potential of gelatin electrospun fibrillar microenvironments. We explored the effect of increasing the DE content from 1 % to 3 % and 5 %, respectively, on the physico-chemical properties of the fibrous scaffolds denoted FG_DE1, FG_DE3, FG_DE5, regarding the aqueous media affinity, stability under simulated physiological conditions, morphology characteristics, and local mechanical properties at the surface. The presence of biosilica generated composite structures with lower swelling degrees and higher stiffness when compared to gelatin fibers. Increasing DE content led to higher Young modulus, while the stability of the protein matrix in PBS, at 37 °C, over 21 was significantly decreased by the presence of diatomite loadings. The best preosteoblast response was obtained for FG_DE3, with enhanced mineralization during the osteogenic differentiation when compared to the control sample without diatomite. 5 % DE in FG_DE5 proved to negatively influence cells' metabolic activity and morphology. Hence, the obtained composite microfibrillar scaffolds might find application as osteoblast-responsive materials for bone tissue engineering.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 The Authors. Published by Elsevier B.V. All rights reserved.)
Databáze: MEDLINE