Alternations in miR-155 and miR-200 serum levels can serve as biomarkers for COVID-19 in the post-mass vaccination era.

Autor: Aboulela A; Medical Research Institute, Microbiology Department, Alexandria University, Alexandria, Egypt., Taha M; Medical Research Institute, Microbiology Department, Alexandria University, Alexandria, Egypt., Ghazal A; Medical Research Institute, Microbiology Department, Alexandria University, Alexandria, Egypt., Baess A; Faculty of Medicine, Chest Diseases Department, Alexandria University, Alexandria, Egypt., Elsheredy A; Medical Research Institute, Microbiology Department, Alexandria University, Alexandria, Egypt. amel.elsheredy@alexu.edu.eg.
Jazyk: angličtina
Zdroj: Molecular biology reports [Mol Biol Rep] 2024 May 25; Vol. 51 (1), pp. 689. Date of Electronic Publication: 2024 May 25.
DOI: 10.1007/s11033-024-09630-2
Abstrakt: Background: Mass vaccination and natural immunity reduced the severity of COVID-19 cases. SARS-CoV-2 ongoing genome variations imply the use of confirmatory serologic biomarkers besides PCR for reliable diagnosis. MicroRNA molecules are intrinsic components of the innate immune system. The expression of miR155-5p and miR200c-3p was previously correlated with SARS-CoV-2 pathogenesis. This case-control study was conducted during the third peak of the COVID-19 pandemic in Egypt and aimed to calculate the accuracy of miR155-5p and miR200c-3p as biomarkers for COVID-19.
Methods and Results: Thirty out of 400 COVID-19 patients at a main University hospital in Alexandria were included in the study along with 20 age-matched healthy controls. Plasma samples were collected for total and differential CBC. Relative quantitation of miR155-5p and miR200c-3p expression from WBCs was done by RT-qPCR. The expression of miR155-5p and miR200c-3p was positively correlated and was significantly downregulated in COVID-19 patients compared to the healthy control group (p ˂ 0.005). Both miR155-5p and miR200c-3p were of 76% and 74% accuracy as diagnostic biomarkers of COVID-19, respectively. Regarding the differentiation between mild and moderate cases, their accuracy was 80% and 70%, respectively.
Conclusions: miR155-5p and miR200c-3p expression can be used to confirm the diagnosis of COVID-19 and discriminate between mild and moderate cases, with a moderate degree of accuracy.
(© 2024. The Author(s), under exclusive licence to Springer Nature B.V.)
Databáze: MEDLINE
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