Touchpad-based immunochromatographic strip for detecting the skin surface proteins.

Autor: Tsukada H; Graduate School of Organic Materials Science, Yamagata University, 4-3-16 Jonan, 992-8510, Yonezawa, Yamagata, Japan., Wako M; Faculty of Engineering, Department of Polymeric and Organic Materials Engineering, Yamagata University, 4-3-16 Jonan, 992-8510, Yonezawa, Yamagata, Japan., Ueda S; Graduate School of Organic Materials Science, Yamagata University, 4-3-16 Jonan, 992-8510, Yonezawa, Yamagata, Japan., Nagamine K; Graduate School of Organic Materials Science, Yamagata University, 4-3-16 Jonan, 992-8510, Yonezawa, Yamagata, Japan; Faculty of Engineering, Department of Polymeric and Organic Materials Engineering, Yamagata University, 4-3-16 Jonan, 992-8510, Yonezawa, Yamagata, Japan. Electronic address: nagamine@yz.yamagata-u.ac.jp.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2024 Sep; Vol. 692, pp. 115575. Date of Electronic Publication: 2024 May 23.
DOI: 10.1016/j.ab.2024.115575
Abstrakt: This study demonstrates, for the first time, the proof-of-concept of a novel immunosensor, a touchpad-based immunochromatographic strip, that non-invasively extracts and detects skin surface proteins. The strip was composed of a nitrocellulose membrane at the center, where a spot of anti-human IgG capture antibody was physically adsorbed. The capture antibody spot was covered with a glass fiber membrane impregnated with phosphate-buffered saline (PBS) to extract skin surface proteins, avoiding direct contact of the human skin with the capture antibodies. Skin surface IgG was detected in two steps: (1) touching the capture antibody via a glass fiber membrane containing PBS, and (2) dipping the strip into the Au-nanoparticle-labeled secondary antibody to visualize the existence of the captured skin surface IgG on the strip. We qualitatively demonstrated that using a very small amount of PBS while maintaining contact with the skin, skin surface proteins can be concentrated and detected, even with a relatively low-sensitivity immunochromatographic chip. This sensor is expected to be a potential biosensor for the non-invasive diagnosis of the integrity of human skin.
Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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