Lipidomic profiling of Drosophila strains Canton-S and white 1118 reveals intraspecific lipid variations in basal metabolic rate.

Autor: Mendoza-Grimau V; Food Lipid Biomarkers and Health Group, Institute of Food Science Research (CIAL, CSIC-UAM), Madrid 28049, Spain., Pérez-Gálvez A; Group of Chemistry and Biochemistry of Pigments, Instituto de la Grasa, CSIC, Sevilla 41013, Spain., Busturia A; Tissue and organ homeostasis, Centro de Biología Molecular Severo Ochoa, (CBMSO, CSIC-UAM), Madrid 28049, Spain., Fontecha J; Food Lipid Biomarkers and Health Group, Institute of Food Science Research (CIAL, CSIC-UAM), Madrid 28049, Spain. Electronic address: j.fontecha@csic.es.
Jazyk: angličtina
Zdroj: Prostaglandins, leukotrienes, and essential fatty acids [Prostaglandins Leukot Essent Fatty Acids] 2024 Feb; Vol. 201, pp. 102618. Date of Electronic Publication: 2024 May 15.
DOI: 10.1016/j.plefa.2024.102618
Abstrakt: Drosophila melanogaster is a well-established model system for studies on lipid metabolism and energy homeostasis. In this study, we identified and quantified the main components of the lipid profile of two widely utilized Drosophila strains, namely Canton-S and white 1118 , under identical experimental conditions. Differences observed between the strains can be attributed to inherent metabolic divergences, thus limiting the influence of confounding factors. Using the comprehensive lipid data acquired, we applied cluster analysis and PLS-DA techniques to ascertain whether the lipidome could effectively differentiate between the strains. Certain lipid features, such as triacylglycerols, polar lipids, and specific sterol components, could be distinguished between flies of both strains regardless of sex. Our results suggest that although Canton-S and white 1118 have similar lipid profiles and distributions, a selected subset of lipids demonstrates clear discriminatory potential between strains, thereby bearing significant implications for planning biological studies using these strains as control references.
Competing Interests: Declaration of Competing Interest The authors declare no conflict of interest.
(Copyright © 2024 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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