Alpha lipoic acid controls degeneration and ensures follicular development in ovine ovarian tissue cultured in vitro.

Autor: Ñaupas LVS; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Gomes FDR; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Ferreira ACA; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Morais SM; Laboratory of Natural Products Chemistry, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Alves DR; Laboratory of Natural Products Chemistry, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Teixeira DIA; Laboratory of Image Diagnosis Applied to Animal Reproduction, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, E, Brazil., Alves BG; Ovid Research Company, Berkeley, CA, United States., Watanabe Y; Vitrogen YVF Biotech, Cravinhos, SP, Brazil., Figueiredo JR; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Tetaping GM; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil., Rodrigues APR; Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, State University of Ceará, Fortaleza, CE, Brazil. Electronic address: aprrodriguespapers@gmail.com.
Jazyk: angličtina
Zdroj: Theriogenology [Theriogenology] 2024 Sep 01; Vol. 225, pp. 55-66. Date of Electronic Publication: 2024 May 19.
DOI: 10.1016/j.theriogenology.2024.05.024
Abstrakt: This study aims to evaluate the effects of adding alpha lipoic acid (ALA) to the in vitro ovarian tissue culture medium, either fresh or after vitrification/warming. For this purpose, 10 ovaries from five adult sheep were used. Each pair of ovaries gave rise to 16 fragments and were randomly distributed into two groups: fresh (n = 8) and vitrified (n = 8). Two fresh fragments were fixed immediately and considered the control, while another six were cultured in vitro for 14 days in the absence; presence of a constant (100 μM/0-14 day) or dynamic (50 μM/day 0-7 and 100 μM/day 8-14) concentration of ALA. As for the vitrified fragments, two were fixed and the other six were cultured in vitro under the same conditions described for the fresh group. All the fragments were subjected to morphological evaluation, follicular development and stromal density (classical histology), DNA fragmentation (TUNEL), senescence (Sudan Black), fibrosis (Masson's Trichome), and endoplasmic reticulum stress (immunofluorescence). Measurements of the antioxidant capacity against the free radicals 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and estradiol (E 2 ) levels in the culture medium was performed. The results showed that in the absence of ALA, in vitro culture of vitrified ovarian fragments showed a significant reduction (P < 0.05) in follicular morphology and increased the presence of senescence and tissue fibrosis (P < 0.05). Dynamic ALA maintained E 2 levels unchanged (P > 0.05) until the end of vitrified ovarian tissue culture and controlled the levels of ABTS and DPPH radicals in fresh or vitrified cultures. Therefore, it is concluded that ALA should be added to the vitrified ovarian tissue in vitro culture medium to reduce the damage that leads to loss of ovarian function. To ensure steroidogenesis during in vitro culture, ALA should be added dynamically (different concentrations throughout culture).
Competing Interests: Declaration of competing interest The authors have no conflict of interest to declare.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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