Biosynthesis of carotenoids in Azospirillum brasilense Cd is mediated via squalene (C30) route.

Autor: Tiwari N; Laboratory of Bacterial Genetics, School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India., Tripathi AK; Laboratory of Bacterial Genetics, School of Biotechnology, Institute of Science, Banaras Hindu University, Varanasi, 221005, India. Electronic address: anil@bhu.ac.in.
Jazyk: angličtina
Zdroj: Biochemical and biophysical research communications [Biochem Biophys Res Commun] 2024 Aug 30; Vol. 722, pp. 150154. Date of Electronic Publication: 2024 May 22.
DOI: 10.1016/j.bbrc.2024.150154
Abstrakt: Azospirillum brasilense is a non-photosynthetic α-Proteobacteria, belongs to the family of Rhodospirillaceae and produces carotenoids to protect itself from photooxidative stress. In this study, we have used Resonance Raman Spectra to show similarity of bacterioruberins of Halobacterium salinarum to that of A. brasilense Cd. To navigate the role of genes involved in carotenoid biosynthesis, we used mutational analysis to inactivate putative genes predicted to be involved in carotenoid biosynthesis in A. brasilense Cd. We have shown that HpnCED enzymes are involved in the biosynthesis of squalene (C30), which is required for the synthesis of carotenoids in A. brasilense Cd. We also found that CrtI and CrtP desaturases were involved in the transformation of colorless squalene into the pink-pigmented carotenoids. This study elucidates role of some genes which constitute very pivotal role in biosynthetic pathway of carotenoid in A. brasilense Cd.
Competing Interests: Declaration of competing interest The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Anil Kumar Tripathi reports financial support and equipment, drugs, or supplies were provided by Banaras Hindu University. Anil Kumar Tripathi reports a relationship with Banaras Hindu University that includes: employment. None If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2024 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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