| Autor: |
Guardado-Fierros BG; Unidad de Tecnología Ambiental, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Normalistas No. 800, Colinas de la Normal, Guadalajara 44270, Jalisco, Mexico., Tuesta-Popolizio DA; Unidad de Tecnología Ambiental, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Normalistas No. 800, Colinas de la Normal, Guadalajara 44270, Jalisco, Mexico., Lorenzo-Santiago MA; Unidad de Tecnología Ambiental, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Normalistas No. 800, Colinas de la Normal, Guadalajara 44270, Jalisco, Mexico., Rubio-Cortés R; Promoción y Fomento de Agave S. de R.L. de C.V., Ignacio Zaragosa #55 C.P., Ixtlahuacán del Río 45260, Jalisco, Mexico., Camacho-Ruíz RM; Unidad de Biotecnología Industrial, CIATEJ, Camino Arenero 127, Zapopan 44270, Jalisco, Mexico., Castañeda-Nava JJ; Unidad de Biotecnología Vegetal, CIATEJ, Camino Arenero 127, Zapopan 44270, Jalisco, Mexico., Gutiérrez-Mora A; Unidad de Biotecnología Vegetal, CIATEJ, Camino Arenero 127, Zapopan 44270, Jalisco, Mexico., Contreras-Ramos SM; Unidad de Tecnología Ambiental, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. (CIATEJ), Normalistas No. 800, Colinas de la Normal, Guadalajara 44270, Jalisco, Mexico. |
| Abstrakt: |
Agave tequilana Weber var. Blue is used as the primary raw material in tequila production due to its fructans (inulin) content. This study evaluates the formulation of a plant-growth-promoting bacteria (PGPB) consortium ( Pseudomonas sp. and Shimwellia sp.) to increase sugars in A. tequilana under field conditions. A total of three doses were tested: low (5 L ha -1 ), medium (10 L ha -1 ), and high (15 L ha -1 ), with a cellular density of 1 × 10 8 CFU mL -1 and one control treatment (without application). Total reducing sugars (TRS), inulin, sucrose, glucose, fructose, and plant growth were measured in agave plants aged 4-5 years at 0 (T0), 3 (T3), 6 (T6), and 12 (T12) months. Yield was recorded at T12. The TRS increased by 3%, and inulin by 5.3% in the high-dose treatment compared to the control at T12. Additionally, a low content of sucrose, glucose, and fructose (approximately 1%) was detected. At T12, the weight of agave heads increased by 31.2% in the medium dose and 22.3% in the high dose compared to the control. The high dose provided a higher inulin content. The A. tequilana plants were five years old and exhibited growth comparable to the standards for 6-7-year-old plants. This study demonstrates a sustainable strategy for tequila production, optimizing the use of natural resources and enhancing industry performance through increased sugar content and yield. |
