Rapid Detection and Quick Characterization of African Swine Fever Virus Using the VolTRAX Automated Library Preparation Platform.

Autor: O'Donnell V; Plum Island Animal Disease Center, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Orient, NY 11957, USA., Pierce JL; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN 37830, USA., Osipenko O; National Bio- and Agro-Defense Facility, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Manhattan, KS 66502, USA., Xu L; Plum Island Animal Disease Center, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Orient, NY 11957, USA., Berninger A; Oak Ridge Institute for Science and Education (ORISE), Oak Ridge, TN 37830, USA., Lakin SM; National Bio- and Agro-Defense Facility, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Manhattan, KS 66502, USA., Barrette RW; Plum Island Animal Disease Center, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Orient, NY 11957, USA., Gladue DP; National Bio- and Agro-Defense Facility, Agricultural Research Service, U.S. Department of Agriculture, Manhattan, KS 66502, USA.; Plum Island Animal Disease Center, Agricultural Research Service, U.S. Department of Agriculture, Orient, NY 11957, USA., Faburay B; Plum Island Animal Disease Center, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Foreign Animal Disease Diagnostic Laboratory, National Veterinary Services Laboratories, Orient, NY 11957, USA.; National Bio- and Agro-Defense Facility, Animal and Plant Health Inspection Service, U.S. Department of Agriculture, Manhattan, KS 66502, USA.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2024 May 05; Vol. 16 (5). Date of Electronic Publication: 2024 May 05.
DOI: 10.3390/v16050731
Abstrakt: African swine fever virus (ASFV) is the causative agent of a severe and highly contagious viral disease affecting domestic and wild swine. The current ASFV pandemic strain has a high mortality rate, severely impacting pig production and, for countries suffering outbreaks, preventing the export of their pig products for international trade. Early detection and diagnosis of ASFV is necessary to control new outbreaks before the disease spreads rapidly. One of the rate-limiting steps to identify ASFV by next-generation sequencing platforms is library preparation. Here, we investigated the capability of the Oxford Nanopore Technologies' VolTRAX platform for automated DNA library preparation with downstream sequencing on Nanopore sequencing platforms as a proof-of-concept study to rapidly identify the strain of ASFV. Within minutes, DNA libraries prepared using VolTRAX generated near-full genome sequences of ASFV. Thus, our data highlight the use of the VolTRAX as a platform for automated library preparation, coupled with sequencing on the MinION Mk1C for field sequencing or GridION within a laboratory setting. These results suggest a proof-of-concept study that VolTRAX is an effective tool for library preparation that can be used for the rapid and real-time detection of ASFV.
Databáze: MEDLINE
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