| Autor: |
Ermolenko E; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia., Baryshnikova N; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia.; Department of Internal Disease of Stomatology Faculty, Pavlov First St-Petersburg State Medical University, 197022 St-Petersburg, Russia.; Laboratory of Medico-Social Problems of Pediatry, St-Petersburg State Pediatric Medical University, 194100 St-Petersburg, Russia., Alekhina G; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia., Zakharenko A; Oncology Department, Pavlov First St-Petersburg State Medical University, 197022 St-Petersburg, Russia., Ten O; North-Western District Scientific and Clinical Center Named after L. G. Sokolov, 194291 St-Petersburg, Russia., Kashchenko V; Department of Faculty Surgery, St-Petersburg State University, 199034 St-Petersburg, Russia.; Beloostrov High Technology Clinic (MMC VT LLC), 188652 Leningrad Region, Russia., Novikova N; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia., Gushchina O; North-Western District Scientific and Clinical Center Named after L. G. Sokolov, 194291 St-Petersburg, Russia., Ovchinnikov T; North-Western District Scientific and Clinical Center Named after L. G. Sokolov, 194291 St-Petersburg, Russia., Morozova A; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia., Ilina A; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia., Karaseva A; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia.; Microbiology Department, St-Petersburg State University, 199034 St-Petersburg, Russia., Tsapieva A; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia., Gladyshev N; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia., Dmitriev A; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia.; Department of Molecular Biotechnology, Saint-Petersburg State Institute of Technology, 190013 St-Petersburg, Russia., Suvorov A; Scientific and Educational Center 'Molecular Bases of Interaction of Microorganisms and Human', World-Class Research Center 'Center for Personalized Medicine', Institute of Experimental Medicine, 197376 St-Petersburg, Russia. |
| Abstrakt: |
Despite great advances in the treatment of oncological diseases, the development of medical technologies to prevent or reduce complications of therapy, in particular, those associated with surgery and the introduction of antibiotics, remains relevant. The aim of this study is to evaluate the effectiveness of the use of autoprobiotics based on indigenous non-pathogenic strains of Enterococcus faecium and Enterococcus hirae as a personalized functional food product (PFFP) in the complex therapy of colorectal cancer (CRC) in the early postoperative period. A total of 36 patients diagnosed with CRC were enrolled in the study. Study group A comprised 24 CRC patients who received autoprobiotic therapy in the early postoperative period, while the control group C included 12 CRC patients without autoprobiotic therapy. Prior to surgery and between days 14 and 16 post-surgery, comprehensive evaluations were conducted on all patients, encompassing the following: stool and gastroenterological complaints analysis, examination of the gut microbiota (bacteriological study, quantitative polymerase chain reaction, metagenome analysis), and analysis of interleukins in the serum. Results: The use of autoprobiotics led to a decrease in dyspeptic complaints after surgery. It was also associated with the absence of postoperative complications, did not cause any side effects, and led to a decrease in the level of pro-inflammatory cytokines (IL-6 and IL-18) in the blood serum. The use of autoprobiotics led to positive changes in the structure of escherichia and enterococci populations, the elimination of Parvomonas micra and Fusobacterium nucleatum , and a decrease in the quantitative content of Clostridium perfringens and Akkermansia muciniphila . Metagenomic analysis (16S rRNA) revealed an increase in alpha diversity. Conclusion: The introduction of autoprobiotics in the postoperative period is a highly effective and safe approach in the complex treatment of CRC. Future studies will allow the discovery of additional fine mechanisms of autoprobiotic therapy and its impact on the digestive, immune, endocrine, and neural systems. |
