| Autor: |
Baranova EN; All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya St. 42, 127550 Moscow, Russia.; N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences, 127276 Moscow, Russia.; Moscow K.A. Timiryazev Agricultural Academy (RSAU-MTAA), Russian State Agrarian University, Timiryazevskaya 49, 127434 Moscow, Russia., Kononenko NV; All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya St. 42, 127550 Moscow, Russia., Lapshin PV; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya St. 35, 127276 Moscow, Russia., Nechaeva TL; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya St. 35, 127276 Moscow, Russia., Khaliluev MR; All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya St. 42, 127550 Moscow, Russia.; Moscow K.A. Timiryazev Agricultural Academy (RSAU-MTAA), Russian State Agrarian University, Timiryazevskaya 49, 127434 Moscow, Russia., Zagoskina NV; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya St. 35, 127276 Moscow, Russia., Smirnova EA; All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya St. 42, 127550 Moscow, Russia.; Biology Faculty, Lomonosov Moscow State University, Leninskie Gory 1, Building 12, 119991 Moscow, Russia.; Department of Biology, MSU-BIT University, Shenzhen 518172, China., Yuorieva NO; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya St. 35, 127276 Moscow, Russia., Raldugina GN; Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya St. 35, 127276 Moscow, Russia., Chaban IA; All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya St. 42, 127550 Moscow, Russia., Kurenina LV; All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya St. 42, 127550 Moscow, Russia., Gulevich AA; All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya St. 42, 127550 Moscow, Russia. |
| Abstrakt: |
ROS-dependent induction of oxidative damage can be used as a trigger initiating genetically determined non-specific protection in plant cells and tissues. Plants are potentially able to withstand various specific (toxic, osmotic) factors of abiotic effects, but do not have sufficient or specific sensitivity to form an adequate effective response. In this work, we demonstrate one of the possible approaches for successful cold acclimation through the formation of effective protection of photosynthetic structures due to the insertion of the heterologous FeSOD gene into the tobacco genome under the control of the constitutive promoter and equipped with a signal sequence targeting the protein to plastid. The increased enzymatic activity of superoxide dismutase in the plastid compartment of transgenic tobacco plants enables them to tolerate the oxidative factor of environmental stresses scavenging ROS. On the other hand, the cost of such resistance is quite high and, when grown under normal conditions, disturbs the arrangement of the intrachloroplastic subdomains leading to the modification of stromal thylakoids, probably significantly affecting the photosynthesis processes that regulate the efficiency of photosystem II. This is partially compensated for by the fact that, at the same time, under normal conditions, the production of peroxide induces the activation of ROS detoxification enzymes. However, a violation of a number of processes, such as the metabolism of accumulation, and utilization and transportation of sugars and starch, is significantly altered, which leads to a shift in metabolic chains. The expected step for further improvement of the applied technology could be both the use of inducible promoters in the expression cassette, and the addition of other genes encoding for hydrogen peroxide-scavenging enzymes in the genetic construct that are downstream in the metabolic chain. |
