| Autor: |
Chik C; Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada., Larroque AL; The Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada., Zhuang Y; Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada., Feinstein S; Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada., Smith DL; Plant Science Department, McDonald Campus, McGill University, Sainte-Anne-de-Bellevue, QC H9X 3V9, Canada., Andonian S; The Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada.; Division of Urology, McGill University, Montreal, QC H4A 3J1, Canada., Ryan AK; Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada.; The Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada.; Department of Pediatrics, McGill University, Montreal, QC H4A 3J1, Canada., Jean-Claude B; The Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada.; Department of Medicine, Division of Medical Oncology, McGill University, Montreal, QC H4A 3J1, Canada., Gupta IR; Department of Human Genetics, McGill University, Montreal, QC H3A 0C7, Canada.; The Research Institute of the McGill University Health Center, Montreal, QC H4A 3J1, Canada.; Department of Pediatrics, McGill University, Montreal, QC H4A 3J1, Canada. |
| Abstrakt: |
Bryophyllum pinnatum (BP) is a medicinal plant used to treat many conditions when taken as a leaf juice, leaves in capsules, as an ethanolic extract, and as herbal tea. These preparations have been chemically analyzed except for decoctions derived from boiled green leaves. In preparation for a clinical trial to validate BP tea as a treatment for kidney stones, we used NMR and MS analyses to characterize the saturation kinetics of the release of metabolites. During boiling of the leaves, (a) the pH decreased to 4.8 within 14 min and then stabilized; (b) regarding organic acids, citric and malic acid were released with maximum release time (t max ) = 35 min; (c) for glycoflavonoids, quercetin 3-O-α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranoside (Q-3O-ArRh), myricetin 3-O-α-L-arabinopyranosyl-(1 → 2)-α-L-rhamnopyranoside (M-3O-ArRh), kappinatoside, myricitrin, and quercitrin were released with t max = 5-10 min; and (d) the total phenolic content (TPC) and the total antioxidant capacity (TAC) reached a t max at 55 min and 61 min, respectively. In summary, 24 g of leaves boiled in 250 mL of water for 61 min ensures a maximal release of key water-soluble metabolites, including organic acids and flavonoids. These metabolites are beneficial for treating kidney stones because they target oxidative stress and inflammation and inhibit stone formation. |
