| Autor: |
Qi LX; Marine College, Shandong University, Weihai 264209, China.; SDU-ANU Joint Science College, Shandong University, Weihai 264209, China., Wang XT; Marine College, Shandong University, Weihai 264209, China., Huang JP; Marine College, Shandong University, Weihai 264209, China., Yue TY; Marine College, Shandong University, Weihai 264209, China., Lu YS; Marine College, Shandong University, Weihai 264209, China.; SDU-ANU Joint Science College, Shandong University, Weihai 264209, China., San DM; Marine College, Shandong University, Weihai 264209, China., Xu YX; Marine College, Shandong University, Weihai 264209, China.; SDU-ANU Joint Science College, Shandong University, Weihai 264209, China., Han YT; Marine College, Shandong University, Weihai 264209, China.; SDU-ANU Joint Science College, Shandong University, Weihai 264209, China., Guo XY; Marine College, Shandong University, Weihai 264209, China.; SDU-ANU Joint Science College, Shandong University, Weihai 264209, China., Xie WD; Marine College, Shandong University, Weihai 264209, China., Zhou YX; Marine College, Shandong University, Weihai 264209, China. |
| Abstrakt: |
To achieve the environmentally friendly and rapid green synthesis of efficient and stable AgNPs for drug-resistant bacterial infection, this study optimized the green synthesis process of silver nanoparticles (AgNPs) using Dihydromyricetin (DMY). Then, we assessed the impact of AgNPs on zebrafish embryo development, as well as their therapeutic efficacy on zebrafish infected with Methicillin-resistant Staphylococcus aureus (MRSA). Transmission electron microscopy (TEM) and dynamic light-scattering (DLS) analyses revealed that AgNPs possessed an average size of 23.6 nm, a polymer dispersity index (PDI) of 0.197 ± 0.0196, and a zeta potential of -18.1 ± 1.18 mV. Compared to other published green synthesis products, the optimized DMY-AgNPs exhibited smaller sizes, narrower size distributions, and enhanced stability. Furthermore, the minimum concentration of DMY-AgNPs required to affect zebrafish hatching and survival was determined to be 25.0 μg/mL, indicating the low toxicity of DMY-AgNPs. Following a 5-day feeding regimen with DMY-AgNP-containing food, significant improvements were observed in the recovery of the gills, intestines, and livers in MRSA-infected zebrafish. These results suggested that optimized DMY-AgNPs hold promise for application in aquacultures and offer potential for further clinical use against drug-resistant bacteria. |
