| Autor: |
Salvante ERG; Doctoral School, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania., Popoiu AV; Emergency Hospital for Children Louis Turcanu, 300011 Timisoara, Romania.; Center of Expertise for Rare Vascular Disease in Children, Louis Turcanu Children Hospital, 300011 Timisoara, Romania., Saxena AK; Department of Pediatric Surgery, Chelsea Children's Hospital, Chelsea and Westminster Hospital NHS Fdn Trust, Imperial College London, London SW10 9NH, UK., Popoiu TA; Doctoral School, Victor Babes University of Medicine and Pharmacy Timisoara, 300041 Timisoara, Romania.; Department III of Functional Sciences, Discipline of Medical Informatics and Biostatistics, 'Victor Babes' University of Medicine and Pharmacy, No. 2 Eftimie Murgu Square, 300041 Timisoara, Romania., Boia ES; Emergency Hospital for Children Louis Turcanu, 300011 Timisoara, Romania.; Center of Expertise for Rare Vascular Disease in Children, Louis Turcanu Children Hospital, 300011 Timisoara, Romania., Cimpean AM; Center of Expertise for Rare Vascular Disease in Children, Louis Turcanu Children Hospital, 300011 Timisoara, Romania.; Department of Microscopic Morphology/Histology, Victor Babes University of Medicine and Pharmacy, 300041 Timisoara, Romania., Rus FS; National Institute of Research for Electrochemistry and Condensed Matter, Aurel Paunescu Podeanu Street 144, 300569 Timisoara, Romania., Dorobantu FR; Department of Neonatology, Faculty of Medicine and Pharmacy, University of Oradea, 410001 Oradea, Romania., Chis M; Department ME2/Rheumatology, Rehabilitation, Physical Medicine and Balneology, Faculty of Medicine, George Emil Palade University of Medicine, Pharmacy, Science, and Technology of Târgu Mureş, 540088 Targu Mures, Romania.; Clinic of Rheumatology, Emergency County Hospital of Târgu Mureş, 540088 Targu Mures, Romania. |
| Abstrakt: |
Type I collagen, prevalent in the extracellular matrix, is biocompatible and crucial for tissue engineering and wound healing, including angiogenesis and vascular maturation/stabilization as required processes of newly formed tissue constructs or regeneration. Sometimes, improper vascularization causes unexpected outcomes. Vascularization failure may be caused by extracellular matrix collagen and non-collagen components heterogeneously. This study compares the angiogenic potential of collagen type I-based scaffolds and collagen type I/glycosaminoglycans scaffolds by using the chick embryo chorioallantoic membrane (CAM) model and IKOSA digital image analysis. Two clinically used biomaterials, Xenoderm (containing type I collagen derived from decellularized porcine extracellular matrix) and a dual-layer collagen sponge (DLC, with a biphasic composition of type I collagen combined with glycosaminoglycans) were tested for their ability to induce new vascular network formation. The AI-based IKOSA app enhanced the research by calculating from stereomicroscopic images angiogenic parameters such as total vascular area, branching sites, vessel length, and vascular thickness. The study confirmed that Xenoderm caused a fast angiogenic response and substantial vascular growth, but was unable to mature the vascular network. DLC scaffold, in turn, produced a slower angiogenic response, but a more steady and organic vascular maturation and stabilization. This research can improve collagen-based knowledge by better assessing angiogenesis processes. DLC may be preferable to Xenoderm or other materials for functional neovascularization, according to the findings. |
