Salvianolic acid A alleviates H 2 O 2 -induced endothelial oxidative injury via miR-204-5p.

Autor: Qiao X; School of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Cao S; School of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Chen S; School of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Guo Y; Hangzhou TCM Hospital Affiliated to Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Chen N; School of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China., Zheng Y; The 903rd Hospital of the People's Liberation Army, Hangzhou, Zhejiang, China. zhengying72@163.com., Jin B; School of Life Science, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, China. jinbo@zcmu.edu.cn.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2024 May 24; Vol. 14 (1), pp. 11931. Date of Electronic Publication: 2024 May 24.
DOI: 10.1038/s41598-024-62556-4
Abstrakt: Oxidative stress induced endothelial dysfunction plays a particularly important role in promoting the development of cardiovascular diseases (CVDs). Salvianolic acid A (SalA) is a water-soluble component of traditional Chinese medicine Salvia miltiorrhiza Bunge with anti-oxidant potency. This study aims to explore the regulatory effect of SalA on oxidative injury using an in vitro model of H 2 O 2 -induced injury in human umbilical vein endothelial cells (HUVECs). In the study, we determined cell viability, the activities of Lactate dehydrogenase (LDH) and Superoxide dismutase (SOD), cell proliferation rate and intracellular reactive oxygen species (ROS). Flow cytometry was used to detect cell apoptosis. Western-blotting was used to evaluate the expression of cell senescence, apoptosis, autophagy and pyroptosis protein factors. The expression level of miRNA was determined by qRT-PCR. Compared with H 2 O 2 -induced HUVECs, SalA promoted cell viability and cell proliferation rate; decreased LDH and ROS levels; and increased SOD activity. SalA also significantly attenuated endothelial senescence, inhibited cell apoptosis, reversed the increase of LC3 II/I ratio and NLRP3 accumulation. Furthermore, miR-204-5p was regulated by SalA. Importantly, miR-204-5p inhibitor had similar effect to that of SalA on H 2 O 2 -induced HUVECs. Our results indicated that SalA could alleviate H 2 O 2 -induced oxidative injury by downregulating miR-204-5p in HUVECs.
(© 2024. The Author(s).)
Databáze: MEDLINE
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